HEM-12: CHANGE OF RBC DEFORMABILITY DURING VARIOUS STAGE HEMORRHAGIC SHOCK

INTRODUCTION:The aim of our work was a red blood cell deformation (rheological marker) at different degrees of severity of experimental hemorrhagic shock. The study of erythrocyte deformability violation and a compulsory monitoring of this physical value deserve more attention of fundamental and applied sciences. METHODS:The modeling of hemorrhagic shock of different stages was performed in the group of anesthetized animals by means of standard blood discharge from the femoral artery (2,5 ml - first stage of shock; 5 ml - second stage of shock; 5 ml - third stage of shock, n = 12). RESULTS:The index of RBC deformability in control group was 2,25 ± 0,03%. Data shows that index deformability decreases at increase in number of hemorrhagical shock’ stage (12% - one subgroup, 13% - second subgroup and 15% - third subgroup). The analysis of the data was performed using statistical programs “Origin 4.1” (Microsoft. Software, Ivc) and Microsoft Excel. All received results was evaluated statistically and significance of differences between mean values were assessed by Studentʼs Criterion. The conditions for conducting the work on animals corresponded to the European Convention on the protection of experimental animals, adopted in 1986 in Strasbourg and for the conducting the experiment the consent of Ethics Committee was obtained.