Angiotensin II and PDGF-BB Stimulate β1-Integrin–Mediated Adhesion and Spreading in Human VSMCs

ABSTRACTβ1-Integrins play an important role for adhesion and spreading of human smooth muscle cells. In the present study we examined the influence of angiotensin II and platelet-derived growth factor (PDGF)-BB on β1-integrin–dependent functions of human smooth muscle cells obtained from iliac arteries. Treatment of these cells with PDGF-BB (20 ng/mL) and Angiotensin II (1 μmol/L) did not change β1-integrin expression up to 48 hours as analyzed by flow cytometry and reverse transcription polymerase chain reaction. β1-integrins predominantly mediated adhesion of human smooth muscle cells to collagen I (79.7±4.4%, P