Abstract 16513: Kynurenine And Spermidine Are Associated With Reduced Skeletal Muscle Function, Expression Of PPARa And Elevated Depression Score In Patients With Heart Failure

IntroductionReduced skeletal muscle function (SMF) and depression are common in patients with HF. The expression level of PPARα in skeletal muscle correlates to SMF. Skeletal muscle-PPARα was shown in animal models to modulate kynurenine (KYN) metabolism, a metabolite of Tryptophan (TRP), and to reduce stress-induced depression. The aim of our current study was to investigate the relationship between SMF, depression and biogenic aminesKYN, Spermidine (SPR) in patients with HF compared to age-matched healthy controls (HC).MethodsWe recruited 55 participants17 HFpEF, 18 HFrEF outpatients and 20 HC prospectively at the University Hospital Jena, Germany(Age71±6 vs. 68 ±9 vs. 66±7 years, p=0.1; sex (m/f)8/9 vs. 3/15 vs. 7/13, p=0.0001; BMI28.7±4.6 vs. 27.9±5.3 vs. 26.4 ±4.2 kg/m, p=0.2). All participants underwent echocardiography, isokinetic SMF tests, muscle biopsies from the vastus lateralis and assessments depression using hospital anxiety and depression scale (HADS). Expression level of PPARα was measured using real-time PCR. Assessment of 188 metabolites in serum was done using mass spectrometry. The current study focuses on SPR, KYN and TRP.ResultsPatients with HFpEF and HFrEF had higher concentrations of SPR0.2±0.02 vs. 0.2±0.05 vs. 0.0±0.0μM, p