Abstract 16082: Changes in the Expression of MicroRNAs From Spontaneously Hypertensive Rats Submitted to Aerobic Swimming Training May Be Involved in Coronary Artery Vascular Remodeling

Abstract 16082: Changes in the Expression of MicroRNAs From Spontaneously Hypertensive Rats Submitted to Aerobic Swimming Training May Be Involved in Coronary Artery Vascular Remodeling

We investigated in Spontaneously Hypertensive Rats (SHR) the expression of microRNAs and coronary artery vascular remodeling after 10 weeks of aerobic swimming training (SHR-t60 min/ day - 4% overload) and compared with the SHR sedentary and normotensive WKY control groups. All animal care and exper...

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Veröffentlicht in:Circulation (New York, N.Y.) N.Y.), 2018-11, Vol.138 (Suppl_1 Suppl 1), p.A16082-A16082
Hauptverfasser: Roque, Fernanda R, Fernandes, Tiago, Cho, Henrique, das Neves, Vander J, Mota, Glória de Fátima A, Rossoni, Luciana V, Oliveira, Edilamar M
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container_issue Suppl_1 Suppl 1
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container_title Circulation (New York, N.Y.)
container_volume 138
creator Roque, Fernanda R
Fernandes, Tiago
Cho, Henrique
das Neves, Vander J
Mota, Glória de Fátima A
Rossoni, Luciana V
Oliveira, Edilamar M
description We investigated in Spontaneously Hypertensive Rats (SHR) the expression of microRNAs and coronary artery vascular remodeling after 10 weeks of aerobic swimming training (SHR-t60 min/ day - 4% overload) and compared with the SHR sedentary and normotensive WKY control groups. All animal care and experimental procedure were in accordance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals and approved by the local ethics committee of the University of São Paulo (n° 2013/01). Structural properties of isolated coronary arteries were studied with a pressure myograph and we observed an increase in wall thickness and wall-to-lumen ratio in SHR group compared with WKY (p
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All animal care and experimental procedure were in accordance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals and approved by the local ethics committee of the University of São Paulo (n° 2013/01). Structural properties of isolated coronary arteries were studied with a pressure myograph and we observed an increase in wall thickness and wall-to-lumen ratio in SHR group compared with WKY (p<0.001). Swimming training increased lumen (p<0.0001), vessel diameter (p<0.0001) and cross-sectional area (p<0.01) when compared to SHR control, but no changes were observed in wall thickness and wall-to-lumen ratio. Using confocal microscopic we found a larger number of vascular smooth muscle cell (VSMC) nuclei in coronary arteries of SHR and SHR-t groups (p<0.05) compared to WKY group. Through a previous analysis using microRNA array of coronary arteries we selected 16 microRNAs that had their expression restored in the SHR-t group when compared to SHR control. These 16 microRNAs plus other 4 with enriched expression in VSMC were analyzed by Real time PCR. We observed that swimming training restored in coronary arteries of SHR the increased expression of microRNAs -92a-3p (p<0.001), -202-3p (p<0.001), -145-5p (p<0.05), -221-3p (p<0.001) and -222-3p (p<0.01). We conducted a bioinformatics study using the miRWalk and DAVID Database for functional analysis of targets genes predicted for the above 5 microRNAs. Among the most important biological terms identified in this analysis we highlight the signaling pathway of Wnt (p value 0.008; Fold enrichment 1.79; EASE SCORE 0.004) and MAPK (p value 0.027; Fold enrichment 1.45; EASE SCORE 0.017). Both signaling pathways play important role in VSMC differentiation and proliferation. Our results suggest that aerobic swimming training regulates microRNAs that may be related to signaling pathways involved in vascular remodeling, inducing different structural changes in coronary arteries of SHR.]]></description><identifier>ISSN: 0009-7322</identifier><identifier>EISSN: 1524-4539</identifier><language>eng</language><publisher>by the American College of Cardiology Foundation and the American Heart Association, Inc</publisher><ispartof>Circulation (New York, N.Y.), 2018-11, Vol.138 (Suppl_1 Suppl 1), p.A16082-A16082</ispartof><rights>2018 by the American College of Cardiology Foundation and the American Heart Association, Inc.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Roque, Fernanda R</creatorcontrib><creatorcontrib>Fernandes, Tiago</creatorcontrib><creatorcontrib>Cho, Henrique</creatorcontrib><creatorcontrib>das Neves, Vander J</creatorcontrib><creatorcontrib>Mota, Glória de Fátima A</creatorcontrib><creatorcontrib>Rossoni, Luciana V</creatorcontrib><creatorcontrib>Oliveira, Edilamar M</creatorcontrib><title>Abstract 16082: Changes in the Expression of MicroRNAs From Spontaneously Hypertensive Rats Submitted to Aerobic Swimming Training May Be Involved in Coronary Artery Vascular Remodeling</title><title>Circulation (New York, N.Y.)</title><description><![CDATA[We investigated in Spontaneously Hypertensive Rats (SHR) the expression of microRNAs and coronary artery vascular remodeling after 10 weeks of aerobic swimming training (SHR-t60 min/ day - 4% overload) and compared with the SHR sedentary and normotensive WKY control groups. All animal care and experimental procedure were in accordance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals and approved by the local ethics committee of the University of São Paulo (n° 2013/01). Structural properties of isolated coronary arteries were studied with a pressure myograph and we observed an increase in wall thickness and wall-to-lumen ratio in SHR group compared with WKY (p<0.001). Swimming training increased lumen (p<0.0001), vessel diameter (p<0.0001) and cross-sectional area (p<0.01) when compared to SHR control, but no changes were observed in wall thickness and wall-to-lumen ratio. Using confocal microscopic we found a larger number of vascular smooth muscle cell (VSMC) nuclei in coronary arteries of SHR and SHR-t groups (p<0.05) compared to WKY group. Through a previous analysis using microRNA array of coronary arteries we selected 16 microRNAs that had their expression restored in the SHR-t group when compared to SHR control. These 16 microRNAs plus other 4 with enriched expression in VSMC were analyzed by Real time PCR. We observed that swimming training restored in coronary arteries of SHR the increased expression of microRNAs -92a-3p (p<0.001), -202-3p (p<0.001), -145-5p (p<0.05), -221-3p (p<0.001) and -222-3p (p<0.01). We conducted a bioinformatics study using the miRWalk and DAVID Database for functional analysis of targets genes predicted for the above 5 microRNAs. Among the most important biological terms identified in this analysis we highlight the signaling pathway of Wnt (p value 0.008; Fold enrichment 1.79; EASE SCORE 0.004) and MAPK (p value 0.027; Fold enrichment 1.45; EASE SCORE 0.017). Both signaling pathways play important role in VSMC differentiation and proliferation. 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All animal care and experimental procedure were in accordance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals and approved by the local ethics committee of the University of São Paulo (n° 2013/01). Structural properties of isolated coronary arteries were studied with a pressure myograph and we observed an increase in wall thickness and wall-to-lumen ratio in SHR group compared with WKY (p<0.001). Swimming training increased lumen (p<0.0001), vessel diameter (p<0.0001) and cross-sectional area (p<0.01) when compared to SHR control, but no changes were observed in wall thickness and wall-to-lumen ratio. Using confocal microscopic we found a larger number of vascular smooth muscle cell (VSMC) nuclei in coronary arteries of SHR and SHR-t groups (p<0.05) compared to WKY group. Through a previous analysis using microRNA array of coronary arteries we selected 16 microRNAs that had their expression restored in the SHR-t group when compared to SHR control. These 16 microRNAs plus other 4 with enriched expression in VSMC were analyzed by Real time PCR. We observed that swimming training restored in coronary arteries of SHR the increased expression of microRNAs -92a-3p (p<0.001), -202-3p (p<0.001), -145-5p (p<0.05), -221-3p (p<0.001) and -222-3p (p<0.01). We conducted a bioinformatics study using the miRWalk and DAVID Database for functional analysis of targets genes predicted for the above 5 microRNAs. Among the most important biological terms identified in this analysis we highlight the signaling pathway of Wnt (p value 0.008; Fold enrichment 1.79; EASE SCORE 0.004) and MAPK (p value 0.027; Fold enrichment 1.45; EASE SCORE 0.017). Both signaling pathways play important role in VSMC differentiation and proliferation. Our results suggest that aerobic swimming training regulates microRNAs that may be related to signaling pathways involved in vascular remodeling, inducing different structural changes in coronary arteries of SHR.]]></abstract><pub>by the American College of Cardiology Foundation and the American Heart Association, Inc</pub></addata></record>
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title Abstract 16082: Changes in the Expression of MicroRNAs From Spontaneously Hypertensive Rats Submitted to Aerobic Swimming Training May Be Involved in Coronary Artery Vascular Remodeling
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