Abstract 14444: Type 2 Diabetic Rat Model With Low Dose Streptozotocin and High Fat Diet: Characterizing Cardiorenal Function and Fibrosis in Diabetic Cardiomyopathy

IntroductionHigh dose streptozotocin (STZ) injection results in pancreatic destruction and a type 1 diabetic (T1DM) rat model. Low dose STZ with high fat diet may represent a type 2 diabetes (T2DM) insulin-resistance model, which would allow for greater translational investigation into cardiorenal complications of T2DM.PurposeThe objective is to establish and characterize cardiorenal function and fibrosis in a type 2 diabetes rat model.MethodsTen week old male Wistar rats were divided into three groupscontrol n=23, type 1 DM n=17 and type 2 DM n=11. Rats in the type 1 DM group were injected STZ at 65 mg/kg intraperitoneally and fed regular diet. Rats in T2DM were injected with two doses of STZ at 35 mg/kg within 24 hours and fed high fat diet. Rats in the control group were injected with citrate buffer (pH 5.0) and fed regular diet. Weights, blood glucose, and 24-hour urine collection were recorded. After 3 months, rats were anesthetized, blood and urine collected, and tissues harvested for histological analysis.ResultsDiabetes was confirmed in the T1DM and T2DM vs control group (plasma glucose 443±19 and 350±11 vs 114±4 mg/dL). However, blood glucose was lower in T2DM vs T1DM (p