Abstract 10909: Effect of Daily CoQ10 on Expression of Anti-Oxidant Proteins by Proteomics in Hibernating Myocardium

IntroductionCoenzyme Q10 (CoQ10) has been shown to reduce oxidant stress in ischemic heart tissue, though it is unclear whether it alters the expression of anti-oxidant proteins within mitochondria from the ischemic territory.HypothesisUsing a swine model of chronic myocardial ischemia, we tested whether daily administration of CoQ10for 4-weeks would enhance the expression of key anti-oxidant proteins by TMT proteomics in mitochondria from the chronically ischemic regions.MethodsTwelve pigs underwent placement of a constrictor around the LAD artery and had reduced regional function at 12 weeks. Pigs were given daily dietary supplements of either CoQ10 (10 mg/kg/day) or placebo and studied with 2-D ECHO 4 weeks later. Animals were sacrificed and mitochondria were isolated from the LAD regions, for analysis of PGC1 alpha by western gels and mitochondrial antioxidant proteins by TMT Proteomics.ResultsAt 12 weeks following instrumentation, regional wall thickening in the LAD region of the Placebo and CoQ10 regions was lower than remote regions (34±7% versus 62±7%; P