Abstract 18059: Microrna-126 and Microrna-132 Abrogates Deleterious Effects of Diabetes-Induced Impaired Angiogenesis

BackgroundDiabetes-induced microvascular abnormalities are implicated in altering cardiac function and structure, a condition referred as diabetic microangiopathy in heart (DMAH). Endothelial-enriched microRNAs (angiomiRs) have been recently demonstrated to play a major role in regulating angiogenesis. However, their pathophysiological role in development of DMAH is elusive.ObjectiveTo investigate the modulation of angiomiRs (miR-126, miR-132) with evolution of DMAH and demonstrate if restoration of angiomiRs can improve functional properties of high glucose treated endothelial cells.Methods & ResultsCardiac tissues were collected from type-2 diabetic mice (BKS.Cg-m+/+Leprdb/J) every 4Wks, from 8 to 32Wks of age. qPCR revealed significant downregulation of miR-126 and -132 starting at 12Wks and continued till 32Wks (miR-1260.30±0.03; miR-1320.22±0.05,p