Abstract 15368: A Fatty-Acid Binding Protein 4 Inhibitor Prevents Proliferation, Migration and Inflammatory Responses in Vascular Smooth Muscle Cells

BackgroundsFatty-acid binding protein (FABP) 4 is an adipocytokine. Since blood FABP4 level is increased in hypertensive subjects, we tested the hypothesis that FABP4 controls key pathological processes for hypertension development, including proliferation, migration and inflammation of vascular smooth muscle cells (SMCs) as well as contractile reactivity.Methods(1) After cultured rat mesenteric arterial SMCs were stimulated with FABP4, proliferation and migration assays and Western blotting were performed. (2) Acute effects of FABP4 on contraction of rat isolated mesenteric artery were examined. (3) After SMCs were stimulated with PDGF-BB or TNF-α in the presence of BMS309403, an FABP4 inhibitor, proliferation and migration assays, Western blotting and qPCR were done. (4) Chronic effects of BMS309403 (5 mg/kg, i.p., 4 weeks) on systolic blood pressure (SBP) in SHR were examined.Results(1) FABP4 (100 ng/ml) alone had no influence on proliferation, migration and inflammatory states (n=5-10). (2) In contrast, FABP4 significantly augmented noradrenaline-induced smooth muscle contraction (n=10, p