Synthesis and Biological Evaluation of p‐Aminophenol Derivatives Applying In Vivo, Ex Vivo, and In Silico Approach

The study aims to synthesize benzamide derivatives of p‐aminophenol (PAP) by reacting with 4‐benzoyl chloride. The reaction of PAP yields four derivatives: P‐1 [N‐(4‐hydroxyphenyl)benzamide], P‐2 [4ˊ‐bromo‐N‐(4‐hydroxyphenyl)benzamide], P‐3 [4ˊ‐nitro‐N‐(4‐hydroxyphenyl)benzamide] and P‐4 [3ˊ,5ˊ‐dintro‐N‐(4‐hydroxyphenyl)benzamide] and evaluation of biological activity. PAP derivatives hot plate analgesic test produced a significant analgesic effect. The higher analgesic activities were observed at 60 min for all derivatives P‐1, P‐2, P‐3, and P‐4 at 47.65, 48.13, 47.08, and 45.47%, respectively. Derivatives of PAP were also shown to have statistically significant (p