Fluorometric determination of ail-trans retinol in rat serum

A rapid, specific, and sensitive fluorometric assay for retinol in rat serum is reported. Serum retinol is extracted into cyclohexane after dilution of the serum sample with aqueous acetonitrile. Sample volumes as small as 0.2 ml can be used with a limiting detectable concentration of 2.5 × 10‐8 M f...

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Veröffentlicht in:	Journal of pharmaceutical sciences 1981-06, Vol.70 (6), p.685-687
Hauptverfasser:	Wu, Su-Chen, Capomacchia, A. C., Price, J. C.
Format:	Artikel
Sprache:	eng
Schlagworte:	all-trans-fluorometric determination in rat serum Fluorometry-determination of all-trans retinol in rat serum Retinol Retinol, all‐trans—fluorometric determination in rat serum Vitamin A-fluorometric determination in rat serum
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Beschreibung
Zusammenfassung:	A rapid, specific, and sensitive fluorometric assay for retinol in rat serum is reported. Serum retinol is extracted into cyclohexane after dilution of the serum sample with aqueous acetonitrile. Sample volumes as small as 0.2 ml can be used with a limiting detectable concentration of 2.5 × 10‐8 M for retinol.
ISSN:	0022-3549 1520-6017
DOI:	10.1002/jps.2600700630