Elektronenmikroskopische Untersuchung an Komplexen von DNA mit der Restriktionsendonuclease SalGI

The binding properties of the type II restriction endonuclease SalGI to the plasmid DNA pGW 10 has been investigated by electron microscopic studies. Samples were spread by the BCA technique. In the presence of magnesium, SalGI binds as dimers and tetramers to the specific recognition site 5′‐G‐T‐C‐G‐A‐C‐3′ and with lower rate to the sequence 5′‐G‐T‐C‐A‐A‐C‐3′, which represents the recognition site of the restriction endonucleases Hind II and Hinc II.