Oxidosqualene Cyclase Second‐Sphere Residues Profoundly Influence the Product Profile

Oxidosqualene Cyclase Second‐Sphere Residues Profoundly Influence the Product Profile

Cycloartenol synthase converts oxidosqualene to the pentacyclic triterpene alcohol cycloartenol. Mutations at His477 dramatically alter the product profile. However, these changes are catalytically irrelevant in the presence of Tyr410Thr and Ile481Val mutations. Modeling studies have established tha...

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Veröffentlicht in:Chembiochem : a European journal of chemical biology 2004-11, Vol.5 (11), p.1581-1585
Hauptverfasser: Lodeiro, Silvia, Segura, Michael J. R., Stahl, Martin, Schulz‐Gasch, Tanja, Matsuda, Seiichi P. T.
Format: Artikel
Sprache:eng
Schlagworte:
enzyme models
mutagenesis
natural products
protein design
steroids
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Zusammenfassung:Cycloartenol synthase converts oxidosqualene to the pentacyclic triterpene alcohol cycloartenol. Mutations at His477 dramatically alter the product profile. However, these changes are catalytically irrelevant in the presence of Tyr410Thr and Ile481Val mutations. Modeling studies have established that His477 is a second‐sphere residue that affects catalysis indirectly through its interactions with the active‐site residue Tyr410.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.200400086