Fluorogenic DNAzyme Probes as Bacterial Indicators

Fluorogenic DNAzyme Probes as Bacterial Indicators

Lighting up bacteria: An RNA‐cleaving fluorescent DNAzyme (RFD) can produce a fluorescent signal in the crude extracellular mixture generated by live bacterial cells (see picture). These DNAzymes cleave a lone RNA linkage (R) embedded in a DNA chain and flanked by nucleotides labeled with a fluoroph...

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Veröffentlicht in:Angewandte Chemie International Edition 2011-04, Vol.50 (16), p.3751-3754
Hauptverfasser: Ali, M. Monsur, Aguirre, Sergio D., Lazim, Hadeer, Li, Yingfu
Format: Artikel
Sprache:eng
Schlagworte:
bacterial detection
biosensors
DNA cleavage
DNAzymes
fluorescence
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Zusammenfassung:Lighting up bacteria: An RNA‐cleaving fluorescent DNAzyme (RFD) can produce a fluorescent signal in the crude extracellular mixture generated by live bacterial cells (see picture). These DNAzymes cleave a lone RNA linkage (R) embedded in a DNA chain and flanked by nucleotides labeled with a fluorophore (F) and a quencher (Q).
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201100477