A Disulfide Cross‐linking Strategy Useful for Studying Ligand‐induced Structural Changes in GPCRs

This chapter contains sections titled: Introduction Methods and approaches General considerations, caveats and troubleshooting Acknowledgements References

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Bibliographische Detailangaben
Hauptverfasser:	Li, Jian Hua, Ward, Stuart D. C, Han, Sung‐Jun, Hamdan, Fadi F, Wess, Jürgen
Format:	Buchkapitel
Sprache:	eng
Schlagworte:	agonist‐induced conformational changes agonist‐promoted disulfide cross‐link formation in double Cys mutant M3 mAChRs cell culture, transfection and membrane preparation disulfide cross‐linking strategy ‐ studying ligand‐induced structural changes in GPCRs G protein‐coupled receptors (GPCRs) ‐ largest group of cell‐surface receptors found in nature monitoring ligand‐induced changes in M3 mAChR structure Protocol 8.3 urea treatment of receptor‐containing membranes Protocol 8.6 Western blotting analysis rendering M3 mAChR in disulfide cross‐linking studies specific double Cys mutant receptor forming intramolecular disulfide bond
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Zusammenfassung:	This chapter contains sections titled: Introduction Methods and approaches General considerations, caveats and troubleshooting Acknowledgements References
DOI:	10.1002/9780470749210.ch8