Cloning and characterization of a gene encoding extracellular metalloprotease from Streptomyces lividans

Cloning and characterization of a gene encoding extracellular metalloprotease from Streptomyces lividans

The prt gene, encoding a protease (Prt) from Streptomyces lividans TK24, was cloned and sequenced. An S. lividans host with plasmid-borne prt secreted 200 μg/ml of a 22-kDa Prt into the culture medium. Prt is classified as a metalloprotease since its activity is significantly inhibited by 1,10-phena...

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Veröffentlicht in:Gene 1992-02, Vol.111 (1), p.125-130
Hauptverfasser: Lichenstein, Henri S., Busse, Leigh A., Smith, Gregory A., Narhi, Linda O., McGinley, Michael O., Rohde, Michael F., Katzowitz, Jessica L., Zukowski, Mark M.
Format: Artikel
Sprache:eng
Schlagworte:
1,10-phenanthroline
[ 14C]α-casein assay
Amino Acid Sequence
Bacterial Proteins
Base Sequence
Biological and medical sciences
Cloning, Molecular
DNA, Bacterial
DNA-binding motif
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genes. Genome
Genetics & Heredity
Genomic Library
Hydrogen-Ion Concentration
Life Sciences & Biomedicine
LysR family
Metalloendopeptidases - genetics
Metalloendopeptidases - metabolism
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
phosphoramidon
plasmid pIJ699
Restriction Mapping
Science & Technology
secretion
Sequence Alignment
signal sequence
Streptomyces - enzymology
Streptomyces - genetics
Streptomyces lividans
Temperature
Zinc-binding motif
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Beschreibung
Zusammenfassung:The prt gene, encoding a protease (Prt) from Streptomyces lividans TK24, was cloned and sequenced. An S. lividans host with plasmid-borne prt secreted 200 μg/ml of a 22-kDa Prt into the culture medium. Prt is classified as a metalloprotease since its activity is significantly inhibited by 1,10-phenanthroline or EDTA. The region upstream from prt codes for an incomplete open reading frame (ORF) oriented opposite to prt. This ORF has a strong similarity to a gene family ( lysR) whose members regulate the transcription of structural genes required for either biosynthesis or degradation.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(92)90613-T