Decreased fidelity of DNA polymerases and decreased DNA excision repair in aging mice: Effects of caloric restriction

Hepatic DNA polymerases from calorie restricted and ad libitum 26 month old C57BL 6 mice showed a decline in fidelity of nucleotide incorporation compared with weanling animals. Both α and β polymerases from calorie restricted aged mice exhibited a higher level of fidelity than polymerases from ad l...

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Veröffentlicht in:Biochemical and biophysical research communications 1992-01, Vol.182 (2), p.712-721
Hauptverfasser: Srivastava, Vinod K., Busbee, David L.
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Sprache:eng
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Zusammenfassung:Hepatic DNA polymerases from calorie restricted and ad libitum 26 month old C57BL 6 mice showed a decline in fidelity of nucleotide incorporation compared with weanling animals. Both α and β polymerases from calorie restricted aged mice exhibited a higher level of fidelity than polymerases from ad libitum aged mice. UV-initiated unscheduled DNA synthesis was significantly higher in hepatocytes from weanling and 18 month old calorie restricted animals compared with cells from 18 month old ad libitum animals, while MMS-initiated unscheduled DNA synthesis did not differ significantly between cells from young and old or ad libitum and calorie restricted animals. These data suggest that calorie restriction could play a significant role in decreasing the age-related decline of cellular mechanisms expected to reduce the rate at which mutations accumulate during aging, and could potentially prolong the onset age of mutation-associated diseases of the elderly.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(92)91790-W