Decreased fidelity of DNA polymerases and decreased DNA excision repair in aging mice: Effects of caloric restriction
Hepatic DNA polymerases from calorie restricted and ad libitum 26 month old C57BL 6 mice showed a decline in fidelity of nucleotide incorporation compared with weanling animals. Both α and β polymerases from calorie restricted aged mice exhibited a higher level of fidelity than polymerases from ad l...
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Veröffentlicht in: | Biochemical and biophysical research communications 1992-01, Vol.182 (2), p.712-721 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Hepatic DNA polymerases from calorie restricted and
ad libitum 26 month old
C57BL
6
mice showed a decline in fidelity of nucleotide incorporation compared with weanling animals. Both α and β polymerases from calorie restricted aged mice exhibited a higher level of fidelity than polymerases from
ad libitum aged mice. UV-initiated unscheduled DNA synthesis was significantly higher in hepatocytes from weanling and 18 month old calorie restricted animals compared with cells from 18 month old
ad libitum animals, while MMS-initiated unscheduled DNA synthesis did not differ significantly between cells from young and old or
ad libitum and calorie restricted animals. These data suggest that calorie restriction could play a significant role in decreasing the age-related decline of cellular mechanisms expected to reduce the rate at which mutations accumulate during aging, and could potentially prolong the onset age of mutation-associated diseases of the elderly. |
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ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1016/0006-291X(92)91790-W |