The Preventive Role of Hydrogen-Rich Water in Thioacetamide-Induced Cholangiofibrosis in Rat Assessed by Automated Histological Classification

Background: Cholangiofibrosis is a controversial intrahepatic cholangial lesion that precedes the development of cholangiocarcinoma. Here, we demonstrate that molecular hydrogen (H-2) can be used to effectively prevent cholangiofibrosis.Methods: The safety and quality of life (QOL) of rats was firstly evaluated. H-2 was administered to rats subjected to thioacetamide (TAA)-induced cholangiofibrosis throughout the whole process. Then, rats were administrated with TAA for 3 months and then followed by H-2 intervention. Rat livers were harvested and assessed by light microscopy and convolutional neural network. RNA-seq was performed to analyze the genetic changes in these animal models.Results: Continuous use of H-2-rich water was safe and improved QOL.The incidence and average number of cholangiofibrosis in the liver were higher in the TAA group (100%, 12.0 +/- 10.07) than that in the H-2 group (57.1%, 2.86 +/- 5.43). The AI algorithm revealed higher Alesion/Aliver in the TAA group (19.6% +/- 9.01) than that in the H-2 group (7.54% +/- 11.0). RNA-seq analysis revealed that H-2 results in a decline in glycolysis. Moreover, in the third experiment, the incidence of microscopic or suspicious tumors and the ratio of liver lesions was decreased after long-term use of H-2 (12.5%, 0.57% +/- 0.45) compared with untreated group (100%, 0.98% +/- 0.73). A number of intestinal microbiota was changed after H-2 usage, including clostridiaceae_1, ruminococcus, turicibacter, coriobacteriales, actinobacteria, and firmicutes_bacterium.Conclusion: Hydrogen-rich water protects against liver injury and cholangiofibrosis and improved quality of life partially through regulating the composition of intestinal flora.