Broad immunophenotyping of the murine brain tumor microenvironment

Here we present a 14-color flow cytometry panel for the evaluation of 13 myeloid and lymphoid populations within murine glioblastoma samples. Reagents, processing protocols, and downstream analyses were thoroughly validated and optimized to resolve the following populations: T cells (CD4, CD8, CD3),...

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Veröffentlicht in:	Journal of immunological methods 2021-12, Vol.499, p.113158-113158, Article 113158
Hauptverfasser:	Tomaszewski, W.H., Waibl-Polania, J., Miggelbrink, A.M., Chakraborty, M.A., Fecci, P.E., Sampson, J.H., Gunn, M.D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biochemical Research Methods Biochemistry & Molecular Biology Brain Neoplasms - diagnostic imaging Brain Neoplasms - immunology Glioblastoma Immunology Immunophenotyping Life Sciences & Biomedicine Mice Mice, Inbred C57BL Multicolor flow cytometry Preclinical Science & Technology Tumor microenvironment Tumor Microenvironment - immunology Unbiased cell classification
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Zusammenfassung:	Here we present a 14-color flow cytometry panel for the evaluation of 13 myeloid and lymphoid populations within murine glioblastoma samples. Reagents, processing protocols, and downstream analyses were thoroughly validated and optimized to resolve the following populations: T cells (CD4, CD8, CD3), B cells (B220), NK cells (NK1.1), neutrophils (Ly6G), classical and non-classical monocytes (Ly6c, CD43), macrophages (F4/80, CD11b), microglia (CD45-lo, CD11b), and dendritic cells (DCs) (CD11c, MHC class II). In addition, this panel leaves Alexa Fluor 488/FITC open for the inclusion of fluorescent reporters or congenic marker staining.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/j.jim.2021.113158