Biochar and jasmonic acid application attenuates antioxidative systems and improves growth, physiology, nutrient uptake and productivity of faba bean (Vicia faba L.) irrigated with saline water

The effect of foliar treatment with jasmonic acid at 0.5 mM (JA) and biochar (15 ton ha−1) as a soil amendment for the faba bean (Vicia faba L. Sakha 4) was studied under salinity conditions. Salt stress led to a significant decrease in leaf numbers, leaf areas and plants, chlorophyll content, relative water content, and yield parameters. In contrast, reactive oxygen species, the proline concentration, level of malondialdehyde, and amount of electrolyte leakage were noticeably increased during both seasons under salt levels of 1500 and 3000 ppm sodium chloride (NaCl). Also, enzyme activities (i.e., of superoxide dismutase, catalase, peroxidase, and glutathione reductase) were increased, especially under a high level of salinity stress (3000 ppm). Application of biochar, jasmonic acid, or biochar + jasmonic acid significantly reduced the catalase, superoxide dismutase, and glutathione reductase activities in salt-stressed plants to values approaching those of the control (unstressed) plants, especially under 1500 ppm of NaCl stress. Biochar and jasmonic acid treatments mitigated the damaging effects of salinity and improved the plant status as indicated by the plant height, leaf area, relative water content, and chlorophyll a and b concentrations. Moreover, biochar and jasmonic acid treatments of the salt-stressed plants enhanced plant productivity, number of flowers, number of seeds per plant, and weight of 100 seeds during two successive seasons. Overall, this study suggests that biochar or jasmonic acid treatments might be promising for mitigating the detrimental impact of salt stress on faba beans. •Saline irrigation becomes an essential practice to sustain food production.•Faba bean is the main source of protein in the Mediterranean Sea region•Application of Jasmonic acid (JA) showed a promising role in the salinity stress tolerance.•Biochar as a soil amendment proved to reduce salinity stress.•Combination of JA and biochar led to improved soil properties and crop production under salinity stress.