Characterization of high-H2O2-tolerant bacterial cytochrome P450 CYP105D18: insights into papaverine N-oxidation

Characterization of high-H2O2-tolerant bacterial cytochrome P450 CYP105D18: insights into papaverine N-oxidation

The bacterial CYP105 family is involved in secondary metabolite biosynthetic pathways and plays essential roles in the biotransformation of xenobiotics. This study investigates the newly identified H2O2-mediated CYP105D18 from Streptomyces laurentii as the first bacterial CYP for N-oxidation. The ca...

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Veröffentlicht in:IUCrJ 2021-07, Vol.8 (4), p.684-694
Hauptverfasser: Pardhe, Bashu Dev, Do, Hackwon, Jeong, Chang-Sook, Kim, Ki-Hwa, Lee, Jun Hyuck, Oh, Tae-Jin
Format: Artikel
Sprache:eng
Schlagworte:
Bacteria
Biotransformation
Chemistry
Chemistry, Multidisciplinary
co-crystals
crystal morphology
Crystallography
cyp105d18
Cytochromes P450
enzyme mechanisms
h2o2 tolerance
Hydrogen peroxide
Materials Science
Materials Science, Multidisciplinary
Metabolites
Oxidation
Oxidation rate
papaverine n-oxide
Physical Sciences
Research Papers
Science & Technology
streptomyces laurentii
Structural analysis
Substrates
Technology
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Zusammenfassung:The bacterial CYP105 family is involved in secondary metabolite biosynthetic pathways and plays essential roles in the biotransformation of xenobiotics. This study investigates the newly identified H2O2-mediated CYP105D18 from Streptomyces laurentii as the first bacterial CYP for N-oxidation. The catalytic efficiency of CYP105D18 for papaverine N-oxidation was 1.43 s(-1) mu M-1. The heme oxidation rate (k) was low (
ISSN:2052-2525
2052-2525
DOI:10.1107/S2052252521005522