Protective effect of naringin in rat model of renal ischemia reperfusion injury

Objective: We aimed to research that naringin whether protects from renal ischemia/reperfusion induced renal damage in rats. Methods: Twenty-four Wistar albino female rats randomly were divided into three groups: 1) control group, in which the rats were only performed right nephrectomy; 2) a second group received right nephrectomy and left kidney ischemia (1 h) and reperfusion (24 h) group ischemia/reperfusion (I/R); 3) a third group received 50 mg/kg naringin orally once a day for two weeks before ischemia/reperfusion (I/R/N). Expression of cyclooxygenase-2 (COX-2), cytosolic phospholipase A 2 (cPLA2), inducible nitric oxide synthase (iNOS), caspase-3, B-cell lymphoma-2 (Bcl-2), Bcl-2 associated x protein (Bax), serum creatinine (Cr), tumor necrosis factor alpha (TNF-alpha), interleukin 6 (IL-6) were measured by using enzyme-linked immunosorbent assay (ELISA). Results: Naringin-treated rats that performed renal ischemia/reperfusion demonstrated significant decrease in Cr, IL-6 and TNF-alpha levels when compared to the only renal ischemia/reperfusion performed rats. While renal ischemia/reperfusion caused a decrease of bcl-2 (1.72 +/- 0.20 pg/ml) levels, while an increase of COX-2 (11882 +/- 642 pg/ml), cPLA2 (2448 +/- 139 pg/ml), iNOS (4331 +/- 438 IU/ml), cleaved caspase-3 (7.33 +/- 0.76 ng/ml) and Bax (2.33 +/- 0.44 ng/ml) levels. The treatment of naringin reversed these kidney effects (7.47 +/- 60.35 pg/ml; 9299 +/- 327 pg/ml; 2001 +/- 78 pg/ml; 3112 +/- 220 IU/ml; 3.38 +/- 0.54 ng/ml; 2.33 +/- 0.44 ng/ml, respectively) (p