GLIPR1 and SPARC expression profile reveals a signature associated with prostate Cancer Brain metastasis

Despite advances in treatment of lethal prostate cancer, the incidence of prostate cancer brain metastases is increasing. In this sense, we analyzed the molecular profile, as well as the functional consequences involved in the reciprocal interactions between prostate tumor cells and human astrocytes. We observed that the DU145 cells, but not the LNCaP cells or the RWPE-1 cells, exhibited more pronounced, malignant and invasive phenotypes along their interactions with astrocytes. Moreover, global gene expression analysis revealed several genes that were differently expressed in our co-culture models with the overexpression of GLIPR1 and SPARC potentially representing a molecular signature associated with the invasion of central nervous system by prostate malignant cells. Further, these results were corroborated by immunohistochemistry and in silico analysis. Thus, we conjecture that the data here presented may increase the knowledge about the molecular mechanisms associated with the invasion of CNS by prostate malignant cells. •Prostate cancer brain metastasis: cellular and molecular basis.•Reciprocal interactions between prostate tumor cells and human astrocytes induce a significant change in the global gene expression profile of tumor cells.•GLIPR1 and SPARC are expressed at higher levels in prostate cancer brain metastasis tissue.•SPARC expression is associated with patients' Gleason score and affect their disease-free survival.