Rationally designed Shewanella oneidensis Biofilm Toilored Graphene-Magnetite Hybrid Nanobiocomposite as Reusable Living Functional Nanomaterial for Effective Removal of Trivalent Chromium

Sustainable treatment of wastewater containing trivalent chromium (Cr3+) remains a significant challenge owing to the several limitations of the existing methodologies. Herein, combination of biosynthesis and Response Surface Methodology (RSM) for the fabrication and optimization of Shewanella oneidensis biofilm functionalized graphene-magnetite (GrM) nanobiocomposite was adopted as a ‘living functional nanomaterial’ (viz. S-GrM) for effective removal of Cr3+ ions from aqueous solution. In the biosynthetic process, S. oneidensis cells reduced the GO-akaganeite complex and adhered on the as-synthesized GrM nanocomposite to form S-GrM hybrid-nanobiocomposite. The process parameters for fabrication of S-GrM hybrid-nanobiocomposite was optimized by RSM based on four responses of easy magnetic separation, biofilm formation along with protein, and carbohydrate contents in extracellular polymeric substances (EPS). The morphology and chemical composition of S-GrM hybrid-nanobiocomposite were investigated using various spectroscopic and microscopic analyses and subsequently explored for removal of Cr3+ ions. The hybrid-nanobiocomposite effectively removed 304.64 ± 14.02 mg/g of Cr3+ at pH 7.0 and 30 °C, which is found to be very high compared to the previously reported values. The high surface area of graphene, biofilm biomass of S. oneidensis and plenty of functional groups provided a unique structure to the S-GrM hybrid-nanobiocomposite for efficient removal of Cr3+ through synergistic interaction. The FTIR and zeta potential studies confirmed that electrostatic and chelation/complexation reaction played key roles in the adsorption process. The fabrication of S-GrM nanobiocomposite thus creates a novel hybrid ‘living functional nanomaterial’ for low cost, recyclable, and sustainable removal of Cr3+ from wastewater. [Display omitted] •Novel S-GrM as a ‘living functional nanomaterial’.•Optimization of S-GrM by combination of bioinspired synthesis and RSM approach.•Removal of Cr3+ ions due to synergistic effect of rGO and S. oneidensis biofilm.•Binding of Cr3+ with S-GrM occurs through electrostatic and complexation reaction. Capsule Summary: Effective removal of trivalent chromium using Shewanella oneidensis biofilm fabricated graphene-magnetite hybrid-nanobiocomposite.