Chronic restraint stress promotes the mobilization and recruitment of myeloid‐derived suppressor cells through β‐adrenergic‐activated CXCL5‐CXCR2‐Erk signaling cascades

Myeloid‐derived suppressor cells (MDSCs) play an important role in tumor immune escape. Recent studies have shown that MDSCs contribute to tumor progression under psychological stress, but the underlying mechanism of MDSCs mobilization and recruitment remains largely unknown. In the present study, a chronic restraint stress paradigm was applied to the H22 hepatocellular carcinoma (HCC) bearing mice to mimic the psychological stress. We observed that chronic restraint stress significantly promoted HCC growth, as well as the mobilization of MDSCs to spleen and tumor sites from bone marrow. Meanwhile, chronic restraint stress enhanced the expression of C‐X‐C motif chemokine receptor 2 (CXCR2) and pErk1/2 in bone marrow MDSCs, together with elevated chemokine (C‐X‐C motif) ligand 5 (CXCL5) expression in tumor tissues. In vitro, the treatments of MDSCs with epinephrine (EPI) and norepinephrine (NE) but not corticosterone (CORT)‐treated H22 conditioned medium obviously inhibited T‐cell proliferation, as well as enhanced CXCR2 expression and extracellular signal‐regulated kinase (Erk) phosphorylation. In vivo, β‐adrenergic blockade with propranolol almost completely reversed the accelerated tumor growth induced by chronic restraint stress and inactivated CXCL5‐CXCR2‐Erk signaling pathway. Our findings support the crucial role of β‐adrenergic signaling cascade in the mobilization and recruitment of MDSCs under chronic restraint stress. What's new? Myeloid derived suppressor cells (MDSCs) contribute to tumor progression under psychological stress, but the underlying mechanism of MDSCs mobilization and recruitment remains largely unknown. Using the chronic restraint stress paradigm in a mouse hepatocellular carcinoma model, here the authors reveal that stress‐induced β‐adrenergic signaling enhances the mobilization and immunosuppressive function of MDSCs via the CXCL5‐CXCR2 axis and Erk signaling activation. These findings support the crucial role of the β‐adrenergic signaling cascade in the harnessing of MDSCs under chronic stress conditions and highlight adrenergic receptor signaling antagonism as a potentially beneficial strategy in cancer therapy.