Aestuariibaculum sediminum sp. nov., a marine bacterium isolated from a tidal flat in Zhoushan

A Gram-staining-negative, non-motile, strictly aerobic bacterium, designated as strain TT11 T , was isolated from a sediment sample of a tidal flat connected in Zhoushan, China. Cells of strain TT11 T are spherical, halotolerant, catalase- and oxidase-positive, and produce carotenoid-like pigments. Colonies were 0.5–1.0 mm diameter, smooth, round, convex and orange-yellow after growth on marine agar at 30 °C for 24 h. Growth of the strain TT11 T was observed at 10–40 °C (optimum, 35 °C), at pH 6.0–9.5 (optimum, pH 6.5), and in the presence of 0–8.0% (w/v) NaCl (optimum, 0.5–1.0%). The results of 16S rRNA gene sequence analysis revealed that strain TT11 T represents a member of the genus Aestuariibaculum and was closely related to Aestuariibaculum suncheonense SC17 T (97.2%) and Aestuariibaculum marinum IP7 T (96.8%). The G + C content of the genome was 34.6%. The only respiratory quinone was MK-6. The major fatty acids (> 10%) were iso-C 15:0 , iso-C 15:1 G and iso-C 17:0 3-OH. The major polar lipids contained phosphatidylethanolamine, phosphoglycolipid, four unidentified aminolipids, four unidentified lipids and two unidentified glycolipids. On the basis of these genomic, chemotaxonomic and phenotypic characteristics, we propose a novel species Aestuariibaculum sediminum sp. nov. with the type strain TT11 T (= KCTC 82195 T = MCCC 1K04734 T ).