α-Particle-induced DNA damage tracks in peripheral blood mononuclear cells of [223Ra]RaCl2-treated prostate cancer patients
Purpose One therapy option for prostate cancer patients with bone metastases is the use of [ 223 Ra]RaCl 2 . The α-emitter 223 Ra creates DNA damage tracks along α-particle trajectories (α-tracks) in exposed cells that can be revealed by immunofluorescent staining of γ-H2AX+53BP1 DNA double-strand b...
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Veröffentlicht in: | European journal of nuclear medicine and molecular imaging 2021-08, Vol.48 (9), p.2761-2770 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Purpose
One therapy option for prostate cancer patients with bone metastases is the use of [
223
Ra]RaCl
2
. The α-emitter
223
Ra creates DNA damage tracks along α-particle trajectories (α-tracks) in exposed cells that can be revealed by immunofluorescent staining of γ-H2AX+53BP1 DNA double-strand break markers. We investigated the time- and absorbed dose-dependency of the number of α-tracks in peripheral blood mononuclear cells (PBMCs) of patients undergoing their first therapy with [
223
Ra]RaCl
2
.
Methods
Multiple blood samples from nine prostate cancer patients were collected before and after administration of [
223
Ra]RaCl
2
, up to 4 weeks after treatment. γ-H2AX- and 53BP1-positive α-tracks were microscopically quantified in isolated and immuno-stained PBMCs.
Results
The absorbed doses to the blood were less than 6 mGy up to 4 h after administration and maximally 16 mGy in total. Up to 4 h after administration, the α-track frequency was significantly increased relative to baseline and correlated with the absorbed dose to the blood in the dose range |
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ISSN: | 1619-7070 1619-7089 |
DOI: | 10.1007/s00259-020-05170-6 |