α-Particle-induced DNA damage tracks in peripheral blood mononuclear cells of [223Ra]RaCl2-treated prostate cancer patients

Purpose One therapy option for prostate cancer patients with bone metastases is the use of [ 223 Ra]RaCl 2 . The α-emitter 223 Ra creates DNA damage tracks along α-particle trajectories (α-tracks) in exposed cells that can be revealed by immunofluorescent staining of γ-H2AX+53BP1 DNA double-strand b...

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Veröffentlicht in:European journal of nuclear medicine and molecular imaging 2021-08, Vol.48 (9), p.2761-2770
Hauptverfasser: Schumann, S., Eberlein, U., Lapa, C., Müller, J., Serfling, S., Lassmann, M., Scherthan, H.
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Sprache:eng
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Zusammenfassung:Purpose One therapy option for prostate cancer patients with bone metastases is the use of [ 223 Ra]RaCl 2 . The α-emitter 223 Ra creates DNA damage tracks along α-particle trajectories (α-tracks) in exposed cells that can be revealed by immunofluorescent staining of γ-H2AX+53BP1 DNA double-strand break markers. We investigated the time- and absorbed dose-dependency of the number of α-tracks in peripheral blood mononuclear cells (PBMCs) of patients undergoing their first therapy with [ 223 Ra]RaCl 2 . Methods Multiple blood samples from nine prostate cancer patients were collected before and after administration of [ 223 Ra]RaCl 2 , up to 4 weeks after treatment. γ-H2AX- and 53BP1-positive α-tracks were microscopically quantified in isolated and immuno-stained PBMCs. Results The absorbed doses to the blood were less than 6 mGy up to 4 h after administration and maximally 16 mGy in total. Up to 4 h after administration, the α-track frequency was significantly increased relative to baseline and correlated with the absorbed dose to the blood in the dose range
ISSN:1619-7070
1619-7089
DOI:10.1007/s00259-020-05170-6