Hydrolyzed polyacrylamide-containing wastewater treatment using ozone reactor-upflow anaerobic sludge blanket reactor-aerobic biofilm reactor multistage treatment system

Polymer flooding is one of the most important enhanced oil recovery techniques. However, a large amount of hydrolyzed polyacrylamide (HPAM)-containing wastewater is produced in the process of polymer flooding, and this poses a potential threat to the environment. In this study, the treatment of HPAM-containing wastewater was analyzed in an ozonic-anaerobic-aerobic multistage treatment process involving an ozone reactor (OR), an upflow anaerobic sludge blanket reactor (UASBR), and an aerobic biofilm reactor (ABR). At an HPAM concentration of 500 mg L−1 and an ozone dose of 25 g O3/g TOC, the HPAM removal rate reached 85.06%. With fracturing of the carbon chain, high-molecular-weight HPAM was degraded into low-molecular-weight compounds. Microbial communities in bioreactors were investigated via high-throughput sequencing, which revealed that norank_c_Bacteroidetes_vadinHA17, norank_f_Cytophagaceae, and Meiothermus were the dominant bacterial groups, and that Methanobacterium, norank_c_WCHA1-57, and Methanosaeta were the key archaeal genera. To the best of our knowledge, this is the first study in which HPAM-containing wastewater is treated using an ozonic-anaerobic-aerobic multistage treatment system. The ideal degradation performance and the presence of keystone microorganisms confirmed that the multistage treatment process is feasible for treatment of HPAM-containing wastewater. [Display omitted] •The OUA multistage treatment system was feasible to treat HPAM-containing wastewater.•The carbon chain of HPAM was completely broken by the multistage process.•Activities of key enzymes involved in HPAM biodegradation was analyzed.•Comprehensive microbial community was investigated by Illumina MiSeq sequencing.•The relationships between key microorganisms and functions of reactors were linked.