Molecular cloning and expression analysis of mc5r like genes (mc5rl) in Ruditapes philippinarum (Manila clam) after aerial exposure and low-temperature stress
The melanocortin-5 receptor (mc5r) plays an important role in exocrine function, lipid metabolism, obesity, and stress response in the vertebrate. However, the functions of the mc5r in mollusks have been rarely investigated. We cloned the full length of Ruditapes philippinarum mc5r like gene ( mc5rl...
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| Veröffentlicht in: | Molecular biology reports 2020-11, Vol.47 (11), p.8891-8901 |
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| description | The melanocortin-5 receptor (mc5r) plays an important role in exocrine function, lipid metabolism, obesity, and stress response in the vertebrate. However, the functions of the
mc5r
in mollusks have been rarely investigated. We cloned the full length of
Ruditapes philippinarum mc5r
like gene (
mc5rl
) and the sequence structure and phylogenetic relationship of
mc5rl
were analyzed
.
Besides, we detected the tissue distribution and the expression pattern of
R. philippinarum mc5r
like (
mc5rl
) genes after aerial exposure and low-temperature stress. The full-length cDNA of the
mc5rl-1
was 2143 bp, consisting of a 1224 bp open reading frame encoding (ORF) 408 amino acids. Sequence and phylogenetic analyses revealed that the nucleotide and amino acid sequences of Manila clam
mc5rl
were highly homologous with
mc5r
of
Crassostrea virginica
,
Crassostrea gigas
,
Mizuhopecten yessoensis
, and
Pecten maximus
(32%-36%) and low homologous with vertebrates. The results of the distribution of
mc5rl
genes showed that
mc5rl
genes were dominant in the mantle, gonad, and hepatopancreas in
R. philippinarum
. The expression of
mc5rl
genes was significantly increased after aerial exposure and low-temperature stress in
R. philippinarum
in hepatopancreas. Aerial exposure and low-temperature stress could induce
mc5rl
expressed.
Mc5rl
might serve as a sensor and promote stress response in
R. philippinarum.
The cloning and expression characteristics of
mc5rl
will facilitate the investigation of its function in stress response and other physiological processes in
R. philippinarum
. |
| doi_str_mv | 10.1007/s11033-020-05941-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_webof</sourceid><recordid>TN_cdi_webofscience_primary_000583107000003</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2456417776</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-7eb6e8158784653e72c3ba01f31dc52489cec10cc8b43a1ee227011ce2b5e6773</originalsourceid><addsrcrecordid>eNqNkc2K1TAUgIMoznX0BVxIwM0dpJqTNE3uUi7-wQyC6LqkuafXjGlSk5ZxXsZnNZ2OI7gQswk5fOcn5yPkKbCXwJh6lQGYEBXjrGJyV0PF75ENSCWqeqf0fbJhgkFVawkn5FHOl4yxGpR8SE6EAK4brTbk50X0aGdvErU-BheO1IQDxR9jwpxdDOVp_HV2mcaeDlYm6t03pEcMmOl2Cfgz6gL9NB_cZMYSHL8678bRBZPmgW4vTHDelOpmOKOmnzBRg8kZvzSJeU5409HHq2rCYcRkpiWWp2WAx-RBb3zGJ7f3Kfny9s3n_fvq_OO7D_vX55UVSk6Vwq5BDVIrXTdSoOJWdIZBL-BgJa_1zqIFZq3uamEAkXPFACzyTmKjlDgl27XumOL3GfPUDi5b9N4EjHNueS2bsjulmoI-_wu9jHMqSyqULESttJCF4itlU8w5Yd-OyQ0mXbfA2sVeu9pri732xl7LS9Kz29JzN-DhLuW3rgK8WIEr7GKfrcNg8Q4rfqUWwBRbjii0_n96X-xNxfc-zmEqqWJNzQUPR0x_PvmP-X8BWx3HMw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2577747835</pqid></control><display><type>article</type><title>Molecular cloning and expression analysis of mc5r like genes (mc5rl) in Ruditapes philippinarum (Manila clam) after aerial exposure and low-temperature stress</title><source>Springer Online Journals Complete</source><source>Web of Science - Science Citation Index Expanded - 2020<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /></source><creator>Jiang, Kunyin ; Nie, Hongtao ; Yan, Xiwu</creator><creatorcontrib>Jiang, Kunyin ; Nie, Hongtao ; Yan, Xiwu</creatorcontrib><description>The melanocortin-5 receptor (mc5r) plays an important role in exocrine function, lipid metabolism, obesity, and stress response in the vertebrate. However, the functions of the
mc5r
in mollusks have been rarely investigated. We cloned the full length of
Ruditapes philippinarum mc5r
like gene (
mc5rl
) and the sequence structure and phylogenetic relationship of
mc5rl
were analyzed
.
Besides, we detected the tissue distribution and the expression pattern of
R. philippinarum mc5r
like (
mc5rl
) genes after aerial exposure and low-temperature stress. The full-length cDNA of the
mc5rl-1
was 2143 bp, consisting of a 1224 bp open reading frame encoding (ORF) 408 amino acids. Sequence and phylogenetic analyses revealed that the nucleotide and amino acid sequences of Manila clam
mc5rl
were highly homologous with
mc5r
of
Crassostrea virginica
,
Crassostrea gigas
,
Mizuhopecten yessoensis
, and
Pecten maximus
(32%-36%) and low homologous with vertebrates. The results of the distribution of
mc5rl
genes showed that
mc5rl
genes were dominant in the mantle, gonad, and hepatopancreas in
R. philippinarum
. The expression of
mc5rl
genes was significantly increased after aerial exposure and low-temperature stress in
R. philippinarum
in hepatopancreas. Aerial exposure and low-temperature stress could induce
mc5rl
expressed.
Mc5rl
might serve as a sensor and promote stress response in
R. philippinarum.
The cloning and expression characteristics of
mc5rl
will facilitate the investigation of its function in stress response and other physiological processes in
R. philippinarum
.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-020-05941-2</identifier><identifier>PMID: 33128687</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Amino acid sequence ; Animal Anatomy ; Animal Biochemistry ; Biochemistry & Molecular Biology ; Biomedical and Life Sciences ; Cloning ; Hepatopancreas ; Histology ; Life Sciences ; Life Sciences & Biomedicine ; Lipid metabolism ; Low temperature ; Mantle ; Melanocortin ; Morphology ; Open reading frames ; Original Article ; Phylogenetics ; Phylogeny ; Ruditapes philippinarum ; Science & Technology ; Stress response</subject><ispartof>Molecular biology reports, 2020-11, Vol.47 (11), p.8891-8901</ispartof><rights>Springer Nature B.V. 2020</rights><rights>Springer Nature B.V. 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>0</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000583107000003</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c375t-7eb6e8158784653e72c3ba01f31dc52489cec10cc8b43a1ee227011ce2b5e6773</citedby><cites>FETCH-LOGICAL-c375t-7eb6e8158784653e72c3ba01f31dc52489cec10cc8b43a1ee227011ce2b5e6773</cites><orcidid>0000-0003-2507-5204</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-020-05941-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-020-05941-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,28253,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33128687$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Kunyin</creatorcontrib><creatorcontrib>Nie, Hongtao</creatorcontrib><creatorcontrib>Yan, Xiwu</creatorcontrib><title>Molecular cloning and expression analysis of mc5r like genes (mc5rl) in Ruditapes philippinarum (Manila clam) after aerial exposure and low-temperature stress</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>MOL BIOL REP</addtitle><addtitle>Mol Biol Rep</addtitle><description>The melanocortin-5 receptor (mc5r) plays an important role in exocrine function, lipid metabolism, obesity, and stress response in the vertebrate. However, the functions of the
mc5r
in mollusks have been rarely investigated. We cloned the full length of
Ruditapes philippinarum mc5r
like gene (
mc5rl
) and the sequence structure and phylogenetic relationship of
mc5rl
were analyzed
.
Besides, we detected the tissue distribution and the expression pattern of
R. philippinarum mc5r
like (
mc5rl
) genes after aerial exposure and low-temperature stress. The full-length cDNA of the
mc5rl-1
was 2143 bp, consisting of a 1224 bp open reading frame encoding (ORF) 408 amino acids. Sequence and phylogenetic analyses revealed that the nucleotide and amino acid sequences of Manila clam
mc5rl
were highly homologous with
mc5r
of
Crassostrea virginica
,
Crassostrea gigas
,
Mizuhopecten yessoensis
, and
Pecten maximus
(32%-36%) and low homologous with vertebrates. The results of the distribution of
mc5rl
genes showed that
mc5rl
genes were dominant in the mantle, gonad, and hepatopancreas in
R. philippinarum
. The expression of
mc5rl
genes was significantly increased after aerial exposure and low-temperature stress in
R. philippinarum
in hepatopancreas. Aerial exposure and low-temperature stress could induce
mc5rl
expressed.
Mc5rl
might serve as a sensor and promote stress response in
R. philippinarum.
The cloning and expression characteristics of
mc5rl
will facilitate the investigation of its function in stress response and other physiological processes in
R. philippinarum
.</description><subject>Amino acid sequence</subject><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biochemistry & Molecular Biology</subject><subject>Biomedical and Life Sciences</subject><subject>Cloning</subject><subject>Hepatopancreas</subject><subject>Histology</subject><subject>Life Sciences</subject><subject>Life Sciences & Biomedicine</subject><subject>Lipid metabolism</subject><subject>Low temperature</subject><subject>Mantle</subject><subject>Melanocortin</subject><subject>Morphology</subject><subject>Open reading frames</subject><subject>Original Article</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Ruditapes philippinarum</subject><subject>Science & Technology</subject><subject>Stress response</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AOWDO</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkc2K1TAUgIMoznX0BVxIwM0dpJqTNE3uUi7-wQyC6LqkuafXjGlSk5ZxXsZnNZ2OI7gQswk5fOcn5yPkKbCXwJh6lQGYEBXjrGJyV0PF75ENSCWqeqf0fbJhgkFVawkn5FHOl4yxGpR8SE6EAK4brTbk50X0aGdvErU-BheO1IQDxR9jwpxdDOVp_HV2mcaeDlYm6t03pEcMmOl2Cfgz6gL9NB_cZMYSHL8678bRBZPmgW4vTHDelOpmOKOmnzBRg8kZvzSJeU5409HHq2rCYcRkpiWWp2WAx-RBb3zGJ7f3Kfny9s3n_fvq_OO7D_vX55UVSk6Vwq5BDVIrXTdSoOJWdIZBL-BgJa_1zqIFZq3uamEAkXPFACzyTmKjlDgl27XumOL3GfPUDi5b9N4EjHNueS2bsjulmoI-_wu9jHMqSyqULESttJCF4itlU8w5Yd-OyQ0mXbfA2sVeu9pri732xl7LS9Kz29JzN-DhLuW3rgK8WIEr7GKfrcNg8Q4rfqUWwBRbjii0_n96X-xNxfc-zmEqqWJNzQUPR0x_PvmP-X8BWx3HMw</recordid><startdate>20201101</startdate><enddate>20201101</enddate><creator>Jiang, Kunyin</creator><creator>Nie, Hongtao</creator><creator>Yan, Xiwu</creator><general>Springer Netherlands</general><general>Springer Nature</general><general>Springer Nature B.V</general><scope>AOWDO</scope><scope>BLEPL</scope><scope>DTL</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2507-5204</orcidid></search><sort><creationdate>20201101</creationdate><title>Molecular cloning and expression analysis of mc5r like genes (mc5rl) in Ruditapes philippinarum (Manila clam) after aerial exposure and low-temperature stress</title><author>Jiang, Kunyin ; Nie, Hongtao ; Yan, Xiwu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-7eb6e8158784653e72c3ba01f31dc52489cec10cc8b43a1ee227011ce2b5e6773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Amino acid sequence</topic><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biochemistry & Molecular Biology</topic><topic>Biomedical and Life Sciences</topic><topic>Cloning</topic><topic>Hepatopancreas</topic><topic>Histology</topic><topic>Life Sciences</topic><topic>Life Sciences & Biomedicine</topic><topic>Lipid metabolism</topic><topic>Low temperature</topic><topic>Mantle</topic><topic>Melanocortin</topic><topic>Morphology</topic><topic>Open reading frames</topic><topic>Original Article</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Ruditapes philippinarum</topic><topic>Science & Technology</topic><topic>Stress response</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Kunyin</creatorcontrib><creatorcontrib>Nie, Hongtao</creatorcontrib><creatorcontrib>Yan, Xiwu</creatorcontrib><collection>Web of Science - Science Citation Index Expanded - 2020</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Kunyin</au><au>Nie, Hongtao</au><au>Yan, Xiwu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and expression analysis of mc5r like genes (mc5rl) in Ruditapes philippinarum (Manila clam) after aerial exposure and low-temperature stress</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><stitle>MOL BIOL REP</stitle><addtitle>Mol Biol Rep</addtitle><date>2020-11-01</date><risdate>2020</risdate><volume>47</volume><issue>11</issue><spage>8891</spage><epage>8901</epage><pages>8891-8901</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>The melanocortin-5 receptor (mc5r) plays an important role in exocrine function, lipid metabolism, obesity, and stress response in the vertebrate. However, the functions of the
mc5r
in mollusks have been rarely investigated. We cloned the full length of
Ruditapes philippinarum mc5r
like gene (
mc5rl
) and the sequence structure and phylogenetic relationship of
mc5rl
were analyzed
.
Besides, we detected the tissue distribution and the expression pattern of
R. philippinarum mc5r
like (
mc5rl
) genes after aerial exposure and low-temperature stress. The full-length cDNA of the
mc5rl-1
was 2143 bp, consisting of a 1224 bp open reading frame encoding (ORF) 408 amino acids. Sequence and phylogenetic analyses revealed that the nucleotide and amino acid sequences of Manila clam
mc5rl
were highly homologous with
mc5r
of
Crassostrea virginica
,
Crassostrea gigas
,
Mizuhopecten yessoensis
, and
Pecten maximus
(32%-36%) and low homologous with vertebrates. The results of the distribution of
mc5rl
genes showed that
mc5rl
genes were dominant in the mantle, gonad, and hepatopancreas in
R. philippinarum
. The expression of
mc5rl
genes was significantly increased after aerial exposure and low-temperature stress in
R. philippinarum
in hepatopancreas. Aerial exposure and low-temperature stress could induce
mc5rl
expressed.
Mc5rl
might serve as a sensor and promote stress response in
R. philippinarum.
The cloning and expression characteristics of
mc5rl
will facilitate the investigation of its function in stress response and other physiological processes in
R. philippinarum
.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>33128687</pmid><doi>10.1007/s11033-020-05941-2</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-2507-5204</orcidid></addata></record> |
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| ispartof | Molecular biology reports, 2020-11, Vol.47 (11), p.8891-8901 |
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| source | Springer Online Journals Complete; Web of Science - Science Citation Index Expanded - 2020<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /> |
| subjects | Amino acid sequence Animal Anatomy Animal Biochemistry Biochemistry & Molecular Biology Biomedical and Life Sciences Cloning Hepatopancreas Histology Life Sciences Life Sciences & Biomedicine Lipid metabolism Low temperature Mantle Melanocortin Morphology Open reading frames Original Article Phylogenetics Phylogeny Ruditapes philippinarum Science & Technology Stress response |
| title | Molecular cloning and expression analysis of mc5r like genes (mc5rl) in Ruditapes philippinarum (Manila clam) after aerial exposure and low-temperature stress |
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