Molecular cloning and expression analysis of mc5r like genes (mc5rl) in Ruditapes philippinarum (Manila clam) after aerial exposure and low-temperature stress
The melanocortin-5 receptor (mc5r) plays an important role in exocrine function, lipid metabolism, obesity, and stress response in the vertebrate. However, the functions of the mc5r in mollusks have been rarely investigated. We cloned the full length of Ruditapes philippinarum mc5r like gene ( mc5rl...
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Veröffentlicht in: | Molecular biology reports 2020-11, Vol.47 (11), p.8891-8901 |
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Zusammenfassung: | The melanocortin-5 receptor (mc5r) plays an important role in exocrine function, lipid metabolism, obesity, and stress response in the vertebrate. However, the functions of the
mc5r
in mollusks have been rarely investigated. We cloned the full length of
Ruditapes philippinarum mc5r
like gene (
mc5rl
) and the sequence structure and phylogenetic relationship of
mc5rl
were analyzed
.
Besides, we detected the tissue distribution and the expression pattern of
R. philippinarum mc5r
like (
mc5rl
) genes after aerial exposure and low-temperature stress. The full-length cDNA of the
mc5rl-1
was 2143 bp, consisting of a 1224 bp open reading frame encoding (ORF) 408 amino acids. Sequence and phylogenetic analyses revealed that the nucleotide and amino acid sequences of Manila clam
mc5rl
were highly homologous with
mc5r
of
Crassostrea virginica
,
Crassostrea gigas
,
Mizuhopecten yessoensis
, and
Pecten maximus
(32%-36%) and low homologous with vertebrates. The results of the distribution of
mc5rl
genes showed that
mc5rl
genes were dominant in the mantle, gonad, and hepatopancreas in
R. philippinarum
. The expression of
mc5rl
genes was significantly increased after aerial exposure and low-temperature stress in
R. philippinarum
in hepatopancreas. Aerial exposure and low-temperature stress could induce
mc5rl
expressed.
Mc5rl
might serve as a sensor and promote stress response in
R. philippinarum.
The cloning and expression characteristics of
mc5rl
will facilitate the investigation of its function in stress response and other physiological processes in
R. philippinarum
. |
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ISSN: | 0301-4851 1573-4978 |
DOI: | 10.1007/s11033-020-05941-2 |