Paw preference is associated with behavioural despair and spatial reference memory in male rats

•Lateralized function is assessed by paw preference in male Wistar rats.•Left-pawed rats are more vulnerable to behavioral despair induced by forced swimming.•Paw preference has no effect on the rate of spatial learning in the Morris water maze.•Left-pawed rats have a significantly better spatial reference memory than right-pawed ones. Paw preference, one of the well-studied behavioural markers of asymmetry, has been associated with affective states and pathologies such as behavioural despair, a rodent model of clinical depression. However, a consistent differential effect of paw preference has not been observed for cognitive functions. In order to investigate the affective properties of paw preference together with its potential cognitive effects, we grouped male Wistar rats as left- or right-pawed, and tested them in the forced swim test and Morris water maze for behavioural despair and spatial memory performance, respectively. We found that left-pawed rats were significantly more susceptible to behavioural despair, while spatial learning performance of the two groups were not different over a five-day Morris water maze task. Left-pawed rats, however, displayed a better reference memory than the right-pawed ones on the subsequent probe trial when the hidden platform of the maze was removed. These findings indicate paw preference as a vulnerability factor for behavioural despair and reveal a previously unknown association between left-paw preference and reference memory performance as assessed in the probe trial of the Morris water maze.