Visualization of Mycotoxins in Living Cells Using Conformation-Resolved Aptamer Nanoprobes

Mycotoxins are the most toxic component and pose a major threat to human health. Monitoring mycotoxins in living cells is essential for their toxicology research; however, tools for the live-cell imaging of mycotoxins remain unexplored. Herein, we proposed a conformation-resolved aptamer nanoprobe (...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:ACS sustainable chemistry & engineering 2020-07, Vol.8 (26), p.9920-9925
Hauptverfasser: Xia, Xuhan, Zhang, Ting, Deng, Sha, Chen, Junbo, Wu, Shijia, He, Chuxian, Zhang, Kaixiang, Deng, Ruijie, He, Qiang
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Mycotoxins are the most toxic component and pose a major threat to human health. Monitoring mycotoxins in living cells is essential for their toxicology research; however, tools for the live-cell imaging of mycotoxins remain unexplored. Herein, we proposed a conformation-resolved aptamer nanoprobe (cresAptamer), allowing us to image mycotoxins in living cells. cresAptamer endows a dual recognition mechanism: binding-induced detachment from nanocarriers and response of intrinsic G-quadruplex of aptamers. The dual recognition mechanism allows us to resolve conformations of aptamer probes corresponding to nonspecific binding and target binding, thus sufficiently supporting the specific identification of target molecules in a complex matrix. cresAptamer significantly improved the signal-to-background ratio for detecting ochratoxin A (OTA), thus allowing us to sensitively and robustly image ochratoxin A in living cells. cresAptamer would be a facile tool for dynamically investigating the toxicology of mycotoxins at the single-cell level.
ISSN:2168-0485
2168-0485
DOI:10.1021/acssuschemeng.0c03399