云南水稻上检测到南方水稻黑条矮缩病毒

Rice samples showing symptoms similar to the disease caused by Southern rice black streaked dwarf virus（SRBSDV,Genus Fijivirus,Family Reoviridae） collected from three municipalities of southern Yunnan were detected by electron microscopy and RT-PCR.Electron microscopy observation of crude infected leaf extracts after negative staining revealed spherical particles of 70-75 nm,and about 411 bp fragment of RT-PCR shared 97.6%-100% nucleotide identities with reported capsid protein gene of SRBSDV isolates.The phylogenetic tree based on the partial capsid protein gene sequences indicated that the virus causing rice dwarf in Yunnan should be identified as SRBSDV.