融合6个组氨酸的人粒细胞-巨噬细胞集落刺激因子在家蚕幼虫中的表达

将融合6个组氨酸（6×his）序列的hGM—CSF基因插入杆状病毒转移载体pBacPAK8中得到杆状病毒重组转移载体pBacPAKHis—GM—CSF，pBacPAKHis—GM—CSFDNA与线性化病毒Bm—BacPAK6DNA共转染BmN细胞，获得了表达rhGM—CSF融合蛋白的重组病毒vBacPAKHis—GM—CSF．用重组病毒感染家蚕五龄起蚕，分别在24、48、72、96，120h和144h剪腹足取蚕血淋巴，ELISA法测得rhGM—CSF融合蛋白在120h的蚕血淋巴中表达量最高，约为15μg／mL蚕血淋巴．融合蛋白通过Poly—His Protein Purification Ki...

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Veröffentlicht in:	Zhejiang daxue xue bao. Journal of Zhejiang University. Agriculture and life sciences. Nong ye yu sheng ming ke xue ban 2005, Vol.31 (5), p.613-616
1. Verfasser:	朱立成陈健林蓉金勇丰张耀洲
Format:	Artikel
Sprache:	chi
Schlagworte:	hGM—CSF 家蚕杆状病毒表达系统融合表达
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container_title	Zhejiang daxue xue bao. Journal of Zhejiang University. Agriculture and life sciences. Nong ye yu sheng ming ke xue ban
container_volume	31
creator	朱立成陈健林蓉金勇丰张耀洲
description	将融合6个组氨酸（6×his）序列的hGM—CSF基因插入杆状病毒转移载体pBacPAK8中得到杆状病毒重组转移载体pBacPAKHis—GM—CSF，pBacPAKHis—GM—CSFDNA与线性化病毒Bm—BacPAK6DNA共转染BmN细胞，获得了表达rhGM—CSF融合蛋白的重组病毒vBacPAKHis—GM—CSF．用重组病毒感染家蚕五龄起蚕，分别在24、48、72、96，120h和144h剪腹足取蚕血淋巴，ELISA法测得rhGM—CSF融合蛋白在120h的蚕血淋巴中表达量最高，约为15μg／mL蚕血淋巴．融合蛋白通过Poly—His Protein Purification Kit纯化．SDS-PAGE和Western blotting分析表明，表达产物是3种糖基化程度不同的，分子量分别约为18、20、31kD的蛋白质．
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Nong ye yu sheng ming ke xue ban</title><addtitle>Journal of Zhejiang University(Agriculture & Life Sciences)</addtitle><description>将融合6个组氨酸（6×his）序列的hGM—CSF基因插入杆状病毒转移载体pBacPAK8中得到杆状病毒重组转移载体pBacPAKHis—GM—CSF，pBacPAKHis—GM—CSFDNA与线性化病毒Bm—BacPAK6DNA共转染BmN细胞，获得了表达rhGM—CSF融合蛋白的重组病毒vBacPAKHis—GM—CSF．用重组病毒感染家蚕五龄起蚕，分别在24、48、72、96，120h和144h剪腹足取蚕血淋巴，ELISA法测得rhGM—CSF融合蛋白在120h的蚕血淋巴中表达量最高，约为15μg／mL蚕血淋巴．融合蛋白通过Poly—His Protein Purification Kit纯化．SDS-PAGE和Western blotting分析表明，表达产物是3种糖基化程度不同的，分子量分别约为18、20、31kD的蛋白质．</description><subject>hGM—CSF</subject><subject>家蚕杆状病毒表达系统</subject><subject>融合表达</subject><issn>1008-9209</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNotTctKw0AAzEHBUvsT3gP7yG6aoxRfUPDSoxCSzaa21hQNYutJsJZehCJ4aCtFb0GtiAeNqdafyTbbv3ChModhhnmsaDkIQFG3ELDWtEIY1lwAEKKYUpjTDuT4RvR7NI2fsmln_hYtruNs2EmTJHu_zaZdeTXWxWckBi9LsRh1Zf9H9JL576UYPYhJX9xH4vVDDu_E17ccPKfxRPXlYyRns3Vt1XcaIS_8c16rbG9VSrt6eX9nr7RZ1hklSGeWzz3PNTgh1CDcIZQbFjEhpNzDnuV4iFGTQkJMAEzCLAJ9jIsGLiLIuDJwXtOXs-dO4DtB1a43z04DdWhf1L1Wy7WDdjs8RgAQBYRUfmOZZ4fNoHpSUw3XYUd-rcFtBJAaJib-A265fTU</recordid><startdate>2005</startdate><enddate>2005</enddate><creator>朱立成陈健林蓉金勇丰张耀洲</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2005</creationdate><title>融合6个组氨酸的人粒细胞-巨噬细胞集落刺激因子在家蚕幼虫中的表达</title><author>朱立成陈健林蓉金勇丰张耀洲</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c652-c9feddb4e55645ea56e4957116ed3d9ad2c67615570075c951f33843821ce75c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2005</creationdate><topic>hGM—CSF</topic><topic>家蚕杆状病毒表达系统</topic><topic>融合表达</topic><toplevel>online_resources</toplevel><creatorcontrib>朱立成陈健林蓉金勇丰张耀洲</creatorcontrib><collection>维普_期刊</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>维普中文期刊数据库</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Zhejiang daxue xue bao. 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identifier	ISSN: 1008-9209
ispartof	Zhejiang daxue xue bao. Journal of Zhejiang University. Agriculture and life sciences. Nong ye yu sheng ming ke xue ban, 2005, Vol.31 (5), p.613-616
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language	chi
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source	EZB-FREE-00999 freely available EZB journals
subjects	hGM—CSF 家蚕杆状病毒表达系统融合表达
title	融合6个组氨酸的人粒细胞-巨噬细胞集落刺激因子在家蚕幼虫中的表达
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