SOD活性和同工酶检测系统中干扰因素的研究

采用NBT光化还原反应检测SOD活性时，反应系统中蛋白质含量在100μg以上将对SOD活性测定造成严重干扰，并且干扰程度随蛋白质含量的增加而加剧；连苯三酚自氧化系统受维生素C的干扰，当反应系统中维生素C的含量在20μg以上，则明显抑制连苯三酚的自氧化。同一植物材料的SOD同工酶在不同浓度的凝胶系统中分离将显示出不同的分离效果。...

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Veröffentlicht in:	Zhejiang daxue xue bao. Journal of Zhejiang University. Agriculture and life sciences. Nong ye yu sheng ming ke xue ban 2002, Vol.28 (2), p.179-182
1. Verfasser:	胡家恕邵爱萍等
Format:	Artikel
Sprache:	chi
Schlagworte:	SOD活性同工酶检测系统干扰因素氮兰四唑超氧物歧化酶连苯三酚
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container_title	Zhejiang daxue xue bao. Journal of Zhejiang University. Agriculture and life sciences. Nong ye yu sheng ming ke xue ban
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creator	胡家恕邵爱萍等
description	采用NBT光化还原反应检测SOD活性时，反应系统中蛋白质含量在100μg以上将对SOD活性测定造成严重干扰，并且干扰程度随蛋白质含量的增加而加剧；连苯三酚自氧化系统受维生素C的干扰，当反应系统中维生素C的含量在20μg以上，则明显抑制连苯三酚的自氧化。同一植物材料的SOD同工酶在不同浓度的凝胶系统中分离将显示出不同的分离效果。
doi_str_mv	10.3321/j.issn:1008-9209.2002.02.015
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subjects	SOD活性同工酶检测系统干扰因素氮兰四唑超氧物歧化酶连苯三酚
title	SOD活性和同工酶检测系统中干扰因素的研究
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