Enhancement of the thermostability of β-1,3-1,4-glucanase by directed evolution

In order to improve the thermostability of β- 1,3-1,4-glucanase, evolutionary molecular engineering was used to evolve the β-1,3-1,4-glucanase from Bacillus subtilis ZJF-1A5. The process involves random mutation by error-prone PCR and DNA shuffling followed by screening on the filter-based assay. Tw...

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Veröffentlicht in:	Journal of Zhejiang University. A. Science 2006-11, Vol.7 (11), p.1948-1955
Hauptverfasser:	Zhang, Xiu-yan, Ruan, Hui, Mu, Lin, He, Guo-qing, Tang, Xing-jun, Chen, Qi-he
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA PCR 耐热性 葡聚糖酶
Online-Zugang:	Volltext
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