Cloning the full—length cDNA of a gene responsible for vascular smooth muscle cell proliferation in atherogenesis and study of function
Objective To clone the full-length cDNA of a gene responsible for vascular smooth muscle cell(v-SMC)proliferation in atherogenesis,and study its function.Methods Oxidized low density lipoprotein(ox-LDL)at optimal concentration was used as the stimulant to induce v-SMC proliferation in culture medium...
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| Veröffentlicht in: | Chinese medical journal 2003-02, Vol.116 (2), p.166-170 |
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| description | Objective To clone the full-length cDNA of a gene responsible for vascular smooth muscle cell(v-SMC)proliferation in atherogenesis,and study its function.Methods Oxidized low density lipoprotein(ox-LDL)at optimal concentration was used as the stimulant to induce v-SMC proliferation in culture medium.A cDNA subtractive library of v-SMC proliferation specific to ox-LDL stimulation was established using subtractive hybridization technique.Methods,including blotting,Northern hybridization and gene sequencing,were used to clone new gene fragments.By using full-length cDNA screening and protein expression techniques,one full-length cDNA was cloned and its function was studied.Results One full-length cDNA was cloned.The new gene(Genbank AF 174647) expressed a 44 kDa protein,which mijht be associated with the activity of ox-LDL.Conclusion The new gene cloned may be associated with SMC proliferation in atherogenesis. |
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Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85656X/85656X.jpg</thumbnail><link.rule.ids>315,781,785</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12775222$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>赵高峰 高鹏 等</creatorcontrib><title>Cloning the full—length cDNA of a gene responsible for vascular smooth muscle cell proliferation in atherogenesis and study of function</title><title>Chinese medical journal</title><addtitle>Chinese Medical Journal</addtitle><description>Objective To clone the full-length cDNA of a gene responsible for vascular smooth muscle cell(v-SMC)proliferation in atherogenesis,and study its function.Methods Oxidized low density lipoprotein(ox-LDL)at optimal concentration was used as the stimulant to induce v-SMC proliferation in culture medium.A cDNA subtractive library of v-SMC proliferation specific to ox-LDL stimulation was established using subtractive hybridization technique.Methods,including blotting,Northern hybridization and gene sequencing,were used to clone new gene fragments.By using full-length cDNA screening and protein expression techniques,one full-length cDNA was cloned and its function was studied.Results One full-length cDNA was cloned.The new gene(Genbank AF 174647) expressed a 44 kDa protein,which mijht be associated with the activity of ox-LDL.Conclusion The new gene cloned may be associated with SMC proliferation in atherogenesis.</description><subject>Amino Acid Sequence</subject><subject>Arteriosclerosis - genetics</subject><subject>Base Sequence</subject><subject>Blotting, Northern</subject><subject>Cell Division - drug effects</subject><subject>Cells, Cultured</subject><subject>Cloning, Molecular</subject><subject>Gene Library</subject><subject>Humans</subject><subject>Lipoproteins, LDL - pharmacology</subject><subject>Molecular Sequence Data</subject><subject>Muscle, Smooth, Vascular - cytology</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Nucleic Acid Hybridization</subject><subject>全长cDNA</subject><subject>动脉粥样硬化</subject><subject>发病机理</subject><subject>基因克隆</subject><subject>血管平滑肌细胞增殖</subject><issn>0366-6999</issn><issn>2542-5641</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkLGO1DAQhiME4paDV0CmoYvk2LEdl6cFDqQTNFBHE8eT9eLYe3YMOipaep6QJ8GrO0QzU8yn79f8j5odEz1rhey7x82OcilbqbW-aJ7lfKSUCaHk0-aiY0oJxtiu-bX3MbiwkO1gCRbv__z87W1YtgMxbz5ekYgEyGKDJcnmUwzZTb6CMZFvkE3xkEheY6z4WrKpJ2O9J6cUvUObYHMxEBcIVH2KZ092mUCYSd7KfHfWYwnmjD1vniD4bF887Mvmy7u3n_fv25tP1x_2VzetYbLfWs0QGTCgU2eR2oGqWQ964oJT0FxIHJAidmD7oR_obHEGqSRqMcyAyiC_bF7fe79DQAjLeIwlhZo4_jiY9cgo5bQO9h-s39wWm7dxdfn8HgQbSx4V511NHSr48gEs02rn8ZTcCulu_NdyBV7dA-YQw3Jb6x4nMF_ReTsqofpu0PwvyTqIhw</recordid><startdate>20030201</startdate><enddate>20030201</enddate><creator>赵高峰 高鹏 等</creator><general>Department of Chest Surgery, The First Hospital of Henan Medical University, Zhengzhou 450052, China%Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20030201</creationdate><title>Cloning the full—length cDNA of a gene responsible for vascular smooth muscle cell proliferation in atherogenesis and study of function</title><author>赵高峰 高鹏 等</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c264t-92ff2a2a0b1ef0e807d989b3530a9356f8f0ff1ae48480defda676f958daf7cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Amino Acid Sequence</topic><topic>Arteriosclerosis - genetics</topic><topic>Base Sequence</topic><topic>Blotting, Northern</topic><topic>Cell Division - drug effects</topic><topic>Cells, Cultured</topic><topic>Cloning, Molecular</topic><topic>Gene Library</topic><topic>Humans</topic><topic>Lipoproteins, LDL - pharmacology</topic><topic>Molecular Sequence Data</topic><topic>Muscle, Smooth, Vascular - cytology</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Nucleic Acid Hybridization</topic><topic>全长cDNA</topic><topic>动脉粥样硬化</topic><topic>发病机理</topic><topic>基因克隆</topic><topic>血管平滑肌细胞增殖</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>赵高峰 高鹏 等</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chinese medical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>赵高峰 高鹏 等</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning the full—length cDNA of a gene responsible for vascular smooth muscle cell proliferation in atherogenesis and study of function</atitle><jtitle>Chinese medical journal</jtitle><addtitle>Chinese Medical Journal</addtitle><date>2003-02-01</date><risdate>2003</risdate><volume>116</volume><issue>2</issue><spage>166</spage><epage>170</epage><pages>166-170</pages><issn>0366-6999</issn><eissn>2542-5641</eissn><abstract>Objective To clone the full-length cDNA of a gene responsible for vascular smooth muscle cell(v-SMC)proliferation in atherogenesis,and study its function.Methods Oxidized low density lipoprotein(ox-LDL)at optimal concentration was used as the stimulant to induce v-SMC proliferation in culture medium.A cDNA subtractive library of v-SMC proliferation specific to ox-LDL stimulation was established using subtractive hybridization technique.Methods,including blotting,Northern hybridization and gene sequencing,were used to clone new gene fragments.By using full-length cDNA screening and protein expression techniques,one full-length cDNA was cloned and its function was studied.Results One full-length cDNA was cloned.The new gene(Genbank AF 174647) expressed a 44 kDa protein,which mijht be associated with the activity of ox-LDL.Conclusion The new gene cloned may be associated with SMC proliferation in atherogenesis.</abstract><cop>China</cop><pub>Department of Chest Surgery, The First Hospital of Henan Medical University, Zhengzhou 450052, China%Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China</pub><pmid>12775222</pmid><tpages>5</tpages></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Arteriosclerosis - genetics Base Sequence Blotting, Northern Cell Division - drug effects Cells, Cultured Cloning, Molecular Gene Library Humans Lipoproteins, LDL - pharmacology Molecular Sequence Data Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - drug effects Nucleic Acid Hybridization 全长cDNA 动脉粥样硬化 发病机理 基因克隆 血管平滑肌细胞增殖 |
| title | Cloning the full—length cDNA of a gene responsible for vascular smooth muscle cell proliferation in atherogenesis and study of function |
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