Cloning the full—length cDNA of a gene responsible for vascular smooth muscle cell proliferation in atherogenesis and study of function

Objective To clone the full-length cDNA of a gene responsible for vascular smooth muscle cell(v-SMC)proliferation in atherogenesis,and study its function.Methods Oxidized low density lipoprotein(ox-LDL)at optimal concentration was used as the stimulant to induce v-SMC proliferation in culture medium.A cDNA subtractive library of v-SMC proliferation specific to ox-LDL stimulation was established using subtractive hybridization technique.Methods,including blotting,Northern hybridization and gene sequencing,were used to clone new gene fragments.By using full-length cDNA screening and protein expression techniques,one full-length cDNA was cloned and its function was studied.Results One full-length cDNA was cloned.The new gene(Genbank AF 174647) expressed a 44 kDa protein,which mijht be associated with the activity of ox-LDL.Conclusion The new gene cloned may be associated with SMC proliferation in atherogenesis.