Temperature Control System for Cell Continuous Observation under the Microscope

This paper developes a tem perature control system in order to carry out a real time and continuous observation on living cells under the m icroscope. In this system, transparent indium tin oxid （ITO） conductive film was used as a heater , tem perature was measured by the tem perature dependence of resistance behavior of the ITO film, C8051F340 single-clup was used as he control hardware core, and the m odified PID algorilhm and pulse width moclubtion （PW M ） were adopted as the key control softw are program s. The tem perature system had a sim ple structure w ithout added tem perature sensor, an uniform tem perature distribution with in ±1℃ on the ITO film surface and tem perature control precision of ±0.2℃ could be obtained. The system cou d m aintain a good grow th state for B H K -21 livhg cell under he microscope for 48 h.