Studies on Biological Characteristics of Rabbit Oral Mucosal Epithelial Cells

Objective：The biological characteristics of rabbit oral mucosal epithelial cells（OMECs） were studied and the suitable procedure for the culture of OMECs was explored.Methods：Cultured OMECs by different surface disinfection,by different Dispase Ⅱ preparation,in different medium,in different calciumion concentration of K-SFM medium.Results：Microbial contamination of cell culture could significantly reduce with 2% iodine solution used for sterilization of oral cavity before cell culture.The OMECs digested with Dispase Ⅱin K-SFM could attach to the culture flask quickly,the OMECs digested with Dispase Ⅱ in PBS rare attached to the culture flask.The proliferations of OMECs cultured in 0 or 0.09 mmol/L calcium concentrations of K-SFM medium showed not statistically significant.the OMECs grew fast with serum,but it was easy to differentiate into fibroblast-like cells.Conclusion：OMECs could grow well and had the typical oral mucosal epithelial cell morphology by the procedure that described in this experiments.