Effects of Soybean Isoflavones on In vitro Antioxidative Capacity of Satellite Cells of Porcine Skeletal Muscles

Effects of Soybean Isoflavones on In vitro Antioxidative Capacity of Satellite Cells of Porcine Skeletal Muscles

S1; A synthetic isoflavone (ISO-S) or genistein was added in culture medium at different concentrations (0,10,20,30,40,and 80 pmol L-1) to investigate the effects of soybean isoflavones on antioxidative capacity of porcine skeletal muscle satellite cells.After 48 h incubation,the suspension was cryo...

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Veröffentlicht in:中国农业科学（英文版） 2011, Vol.10 (1), p.120-125
Hauptverfasser: JIANG Zong-yong, ZHOU Gui-lian, LIN Ying-cai, JIANG Shou-qun, ZHENG Chun-tian, HAN Li-ming, CHEN Fang
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container_title 中国农业科学（英文版）
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ZHOU Gui-lian
LIN Ying-cai
JIANG Shou-qun
ZHENG Chun-tian
HAN Li-ming
CHEN Fang
description S1; A synthetic isoflavone (ISO-S) or genistein was added in culture medium at different concentrations (0,10,20,30,40,and 80 pmol L-1) to investigate the effects of soybean isoflavones on antioxidative capacity of porcine skeletal muscle satellite cells.After 48 h incubation,the suspension was cryopreserved for the determination of superoxide dismutase (SOD),catalase (CAT),glutathione peroxidase (GSH-Px) activities,and malondialdehyde (MDA) content.The mRNA levels of SOD,CAT,and GSH-Px gene in cells were detected with Taqman fluorescent probe method.The results showed that the content of MDA and the activities and the mRNA levels of SOD of porcine skeletal muscle satellite cells were influenced by supplemented soybean isoflavone (P
doi_str_mv 10.1016/S1671-2927(09)60297-2
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A synthetic isoflavone (ISO-S) or genistein was added in culture medium at different concentrations (0,10,20,30,40,and 80 pmol L-1) to investigate the effects of soybean isoflavones on antioxidative capacity of porcine skeletal muscle satellite cells.After 48 h incubation,the suspension was cryopreserved for the determination of superoxide dismutase (SOD),catalase (CAT),glutathione peroxidase (GSH-Px) activities,and malondialdehyde (MDA) content.The mRNA levels of SOD,CAT,and GSH-Px gene in cells were detected with Taqman fluorescent probe method.The results showed that the content of MDA and the activities and the mRNA levels of SOD of porcine skeletal muscle satellite cells were influenced by supplemented soybean isoflavone (P&lt;0.05) when adding 10-80 gmol L-1 ISO-S or genistein in the medium.The MDA contents,SOD and CAT activities and their mRNA expression levels of porcine skeletal muscle cells responded quadratically P(&lt;0.05) as the level of ISO-S or genistein increased.Pre-incubation of porcine skeletal muscle satellite cells with ISO-Sor genistein at 10-40 pmol L-t elevated the activities and the mRNA expression levels of SOD and CAT in cells concurrently and decreased the cellular content of MDA (P&lt;0.05).The results indicated that pre-incubation of ISO-S or genistein at 10-40 pmol L-1 could improve the antioxidative capacity of porcine skeletal muscle satellite cells.</abstract><pub>Key Laboratory of Animal Nutrition and Feed Science South China,Ministry of Agriculture/Institute of Animal Science,Guangdong Academy of Agricultural Science,Guangzhou 510640,P.R.China</pub><doi>10.1016/S1671-2927(09)60297-2</doi></addata></record>
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title Effects of Soybean Isoflavones on In vitro Antioxidative Capacity of Satellite Cells of Porcine Skeletal Muscles
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