Effects of Soybean Isoflavones on In vitro Antioxidative Capacity of Satellite Cells of Porcine Skeletal Muscles

S1; A synthetic isoflavone (ISO-S) or genistein was added in culture medium at different concentrations (0,10,20,30,40,and 80 pmol L-1) to investigate the effects of soybean isoflavones on antioxidative capacity of porcine skeletal muscle satellite cells.After 48 h incubation,the suspension was cryopreserved for the determination of superoxide dismutase (SOD),catalase (CAT),glutathione peroxidase (GSH-Px) activities,and malondialdehyde (MDA) content.The mRNA levels of SOD,CAT,and GSH-Px gene in cells were detected with Taqman fluorescent probe method.The results showed that the content of MDA and the activities and the mRNA levels of SOD of porcine skeletal muscle satellite cells were influenced by supplemented soybean isoflavone (P