Hairy roots induced by Agrobacterium rhizogenes and production of regenerative plants in hairy root cultures in maize
Hairy roots of maize were induced by infecting 15-d calli with Agrobacterium rhizogenes. The hairy roots cultured in hormone-free media showed the vigorous growth and typical hairy root features. The regenerated plants were produced from hairy roots in MS media supplemented with 1.6 mg/L ZT and 0.4...
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Veröffentlicht in: | Science China. Life sciences 2006-08, Vol.49 (4), p.305-310 |
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Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Hairy roots of maize were induced by infecting 15-d calli with Agrobacterium rhizogenes. The hairy roots cultured in hormone-free media showed the vigorous growth and typical hairy root features. The regenerated plants were produced from hairy roots in MS media supplemented with 1.6 mg/L ZT and 0.4 mg/L NAA. The PCR-Southern hybridization demonstrated that T-DNA had been integrated into the chromosome of regenerated plants. |
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ISSN: | 1006-9305 1674-7305 1862-2798 1869-1889 |
DOI: | 10.1007/s11427-006-0305-1 |