Exploring three-dimensional biological tissues with new chemical labels

Three-dimensional (3D) histology has exhibited tremendous potential in fundamental research and clinical disease grading, but compatible labeling techniques are still lacking. Recently in Science Advances, Pac et al. report a new histological technique termed 3DNFC, which realizes 3D fluorescence imaging of thick tissues via citrate-based in situ fluorophore formation. A novel three-dimensional histological technique is highlighted and a citrate-based in situ fluorophore formation approach for fluorescence imaging is introduced. [Display omitted]