An ESIPT-based NIR-fluorescent probe for exosome labeling and in situ imaging

Exosomes play significant roles in physiological and tumorigenic processes and it is desirable to visualize and track the exosomes. Herein, a novel amphiphilic fluorescent probe HBT-Exo based on excited-state intramolecular proton transfer (ESIPT) mechanism is reported for exosome-labeling. Its ESIP...

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Veröffentlicht in:Chinese chemical letters 2023-11, Vol.34 (11), p.108273-224, Article 108273
Hauptverfasser: Ding, Jipeng, Xiao, Runsha, Bi, Anyao, Chen, Guanyang, Zhang, Nengwei, Chen, Zihua, Feng, Xueping, Zeng, Wenbin
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Sprache:eng
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Zusammenfassung:Exosomes play significant roles in physiological and tumorigenic processes and it is desirable to visualize and track the exosomes. Herein, a novel amphiphilic fluorescent probe HBT-Exo based on excited-state intramolecular proton transfer (ESIPT) mechanism is reported for exosome-labeling. Its ESIPT characteristics were confirmed by both theory calculation and experimental observation, which enable the probe to show a large Stokes shift as well as near-infrared (NIR) keto-form emission. HBT-Exo displayed excellent biocompatibility and remarkable efficiency for exosome-labeling in gastric cancer cells. Furthermore, the labeled exosomes were successfully applied for the real-time in situ imaging in mouse models. Schematic illustration of HBT-Exo as a fluorescence exosome-labeling tool. [Display omitted]
ISSN:1001-8417
1878-5964
DOI:10.1016/j.cclet.2023.108273