Recent developments of droplets-based microfluidics for bacterial analysis

Droplet-based microfluidics enables the generation of uniform microdroplets at picoliter or nanoliter scale with high frequency (∼kHz) under precise control. The droplets can function as bioreactors for versatile chemical/biological study and analysis. Taking advantage of the discrete compartment with a confined volume, (1) isolation and manipulation of a single cell, (2) improvement of in-droplet effective concentrations, (3) elimination of heterogeneous population effects, (4) diminution of contamination risks can be achieved, making it a powerful tool for rapid, sensitive, and high-throughput detection and analysis of bacteria, even for rare or unculturable strains in conventional methods. This mini-review will focus on the generation and manipulation of micro-droplets and bacteria detection and analysis carried out by droplet-based microfluidics. Finally, applications with high potential of droplet-based bacteria analysis are briefly introduced. Due to the advantages of rapid, sensitive, high throughput, and compatibility with rare and unculturable bacteria in conventional methods, droplet-based microfluidics has tremendous potential of providing novel solutions for biological medicine, microbiological engineering, environmental ecology, etc. Droplet-based micro-fluidics is a promising analytical technology in featuring small-sample, high-throughput, highly sensitive, and timesaving, etc. This mini-review article introduced and summarized recent advances on the micro-droplets’ generation and manipulation in micro-fluidics for bacteria detection and analysis, and their potential applications. [Display omitted]