Clickable rhodamine spirolactam based spontaneously blinking probe for super-resolution imaging

Spontaneously blinking probe, which switches between dark and bright state without UV or external additives, is extremely attractive in super resolution imaging of live cells. Herein, a clickable rhodamine spirolactam probe, Atto565-Tet, is rationally constructed for spontaneously blinking after biorthogonal labelling and successfully applied to super resolution imaging of mitochondria and lysosomes. Atto565-Tet with the desirable pKa value for spontaneously blinking after biorthogonal labelling was developed and successfully applied to super resolution imaging of mitochondria and lysosomes. [Display omitted]