Relationship between G-CSF and hyperleukocytosis in patients with APL after treatment with all-trans retinoic acid
R73; Objective: To detect the G-CSF effect and its mechanism in hyperleucocytosis of patients with APL after treatment with all-trans retinoic acid (ATRA).Methods: MTT method was used to measure the proliferation of HL-60 cells or primary APL cells.cDNA-mRNA dot blot hybridization was used to detect...
Gespeichert in:
Veröffentlicht in: | Chinese journal of cancer research 1999-12, Vol.11 (4), p.249-252 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 252 |
---|---|
container_issue | 4 |
container_start_page | 249 |
container_title | Chinese journal of cancer research |
container_volume | 11 |
creator | Guosheng, Jiang Tianhua, Tang Guanlin, Sun Yukun, Zhang Wen, Wu Weili, Wu Haiquan, Ren Liangyu, Zhao |
description | R73; Objective: To detect the G-CSF effect and its mechanism in hyperleucocytosis of patients with APL after treatment with all-trans retinoic acid (ATRA).Methods: MTT method was used to measure the proliferation of HL-60 cells or primary APL cells.cDNA-mRNA dot blot hybridization was used to detect the c-myc gene expression level of HL-60 cells after ATRA treatment. Cycle shift of HL-60 cells was measured by flow cytometric Analysis. Results: ATRA could inhibit obviously in vitro the proliferation of HL60 cells of primary APL cells. The G0/G1 ratio of HL-60at 24 hours or 96 hours post-treatment with ATRA was increased, proliferation of HL-60 cells at the same time was promoted obviously under the condition of rhGCSF. C-myc gene expression was analyzed by dot blot hybridization; its expression levels of 8, 24 or 96 hours in culture with ATRA separately were higher, low, high or lower as compared with each other. Conclusion: GCSF plays an important role in hyperleucocytosis of patients with APL after treatment with ATRA. |
doi_str_mv | 10.1007/s11670-999-0026-7 |
format | Article |
fullrecord | <record><control><sourceid>wanfang_jour_cross</sourceid><recordid>TN_cdi_wanfang_journals_zgazyj199904004</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><wanfj_id>zgazyj199904004</wanfj_id><sourcerecordid>zgazyj199904004</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1904-85a5a3d5a8191bd883056b89ea3aae78756650baab51a1364a37047a1fcea6f63</originalsourceid><addsrcrecordid>eNotkF9LwzAUxYsoOKcfwLe8-RS9MU3SPo7hpjBQ_PMcbtt0y-zSkWSM7tObUZ_ugXPOPfDLsnsGjwxAPQXGpAJaliUFeJZUXWQTVpacguTsMmmA5ErIr7ObELYAQglgk8x_mg6j7V3Y2D2pTDwa48iSzr8WBF1DNsPe-M4cfvt6iH2wgVhH9qlhXAzkaOOGzD5WBNtoPIneYNwlZzSw62j06ALxJlrX25pgbZvb7KrFLpi7_zvNfhYv3_NXunpfvs1nK1qzEnJaCBTIG4EFK1nVFAUHIauiNMgRjSqUkFJAhVgJhozLHLmCXCFra4OylXyaPYx_j-hadGu97Q_epUV9WuNp2CY8aQcgT0k2Jmvfh-BNq_fe7tAPmoE-49UjXp0a-oxXK_4HzPtuww</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Relationship between G-CSF and hyperleukocytosis in patients with APL after treatment with all-trans retinoic acid</title><source>SpringerLink Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Guosheng, Jiang ; Tianhua, Tang ; Guanlin, Sun ; Yukun, Zhang ; Wen, Wu ; Weili, Wu ; Haiquan, Ren ; Liangyu, Zhao</creator><creatorcontrib>Guosheng, Jiang ; Tianhua, Tang ; Guanlin, Sun ; Yukun, Zhang ; Wen, Wu ; Weili, Wu ; Haiquan, Ren ; Liangyu, Zhao</creatorcontrib><description>R73; Objective: To detect the G-CSF effect and its mechanism in hyperleucocytosis of patients with APL after treatment with all-trans retinoic acid (ATRA).Methods: MTT method was used to measure the proliferation of HL-60 cells or primary APL cells.cDNA-mRNA dot blot hybridization was used to detect the c-myc gene expression level of HL-60 cells after ATRA treatment. Cycle shift of HL-60 cells was measured by flow cytometric Analysis. Results: ATRA could inhibit obviously in vitro the proliferation of HL60 cells of primary APL cells. The G0/G1 ratio of HL-60at 24 hours or 96 hours post-treatment with ATRA was increased, proliferation of HL-60 cells at the same time was promoted obviously under the condition of rhGCSF. C-myc gene expression was analyzed by dot blot hybridization; its expression levels of 8, 24 or 96 hours in culture with ATRA separately were higher, low, high or lower as compared with each other. Conclusion: GCSF plays an important role in hyperleucocytosis of patients with APL after treatment with ATRA.</description><identifier>ISSN: 1000-9604</identifier><identifier>EISSN: 1993-0631</identifier><identifier>DOI: 10.1007/s11670-999-0026-7</identifier><language>eng</language><publisher>Shangdong Academy of Medical Sciences, Jinan 250062, China%Rui-Jin Hospital, Shanghai Institute of Hematology, Shanghai 200025, China</publisher><ispartof>Chinese journal of cancer research, 1999-12, Vol.11 (4), p.249-252</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1904-85a5a3d5a8191bd883056b89ea3aae78756650baab51a1364a37047a1fcea6f63</citedby><cites>FETCH-LOGICAL-c1904-85a5a3d5a8191bd883056b89ea3aae78756650baab51a1364a37047a1fcea6f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/zgazyj/zgazyj.jpg</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Guosheng, Jiang</creatorcontrib><creatorcontrib>Tianhua, Tang</creatorcontrib><creatorcontrib>Guanlin, Sun</creatorcontrib><creatorcontrib>Yukun, Zhang</creatorcontrib><creatorcontrib>Wen, Wu</creatorcontrib><creatorcontrib>Weili, Wu</creatorcontrib><creatorcontrib>Haiquan, Ren</creatorcontrib><creatorcontrib>Liangyu, Zhao</creatorcontrib><title>Relationship between G-CSF and hyperleukocytosis in patients with APL after treatment with all-trans retinoic acid</title><title>Chinese journal of cancer research</title><description>R73; Objective: To detect the G-CSF effect and its mechanism in hyperleucocytosis of patients with APL after treatment with all-trans retinoic acid (ATRA).Methods: MTT method was used to measure the proliferation of HL-60 cells or primary APL cells.cDNA-mRNA dot blot hybridization was used to detect the c-myc gene expression level of HL-60 cells after ATRA treatment. Cycle shift of HL-60 cells was measured by flow cytometric Analysis. Results: ATRA could inhibit obviously in vitro the proliferation of HL60 cells of primary APL cells. The G0/G1 ratio of HL-60at 24 hours or 96 hours post-treatment with ATRA was increased, proliferation of HL-60 cells at the same time was promoted obviously under the condition of rhGCSF. C-myc gene expression was analyzed by dot blot hybridization; its expression levels of 8, 24 or 96 hours in culture with ATRA separately were higher, low, high or lower as compared with each other. Conclusion: GCSF plays an important role in hyperleucocytosis of patients with APL after treatment with ATRA.</description><issn>1000-9604</issn><issn>1993-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNotkF9LwzAUxYsoOKcfwLe8-RS9MU3SPo7hpjBQ_PMcbtt0y-zSkWSM7tObUZ_ugXPOPfDLsnsGjwxAPQXGpAJaliUFeJZUXWQTVpacguTsMmmA5ErIr7ObELYAQglgk8x_mg6j7V3Y2D2pTDwa48iSzr8WBF1DNsPe-M4cfvt6iH2wgVhH9qlhXAzkaOOGzD5WBNtoPIneYNwlZzSw62j06ALxJlrX25pgbZvb7KrFLpi7_zvNfhYv3_NXunpfvs1nK1qzEnJaCBTIG4EFK1nVFAUHIauiNMgRjSqUkFJAhVgJhozLHLmCXCFra4OylXyaPYx_j-hadGu97Q_epUV9WuNp2CY8aQcgT0k2Jmvfh-BNq_fe7tAPmoE-49UjXp0a-oxXK_4HzPtuww</recordid><startdate>199912</startdate><enddate>199912</enddate><creator>Guosheng, Jiang</creator><creator>Tianhua, Tang</creator><creator>Guanlin, Sun</creator><creator>Yukun, Zhang</creator><creator>Wen, Wu</creator><creator>Weili, Wu</creator><creator>Haiquan, Ren</creator><creator>Liangyu, Zhao</creator><general>Shangdong Academy of Medical Sciences, Jinan 250062, China%Rui-Jin Hospital, Shanghai Institute of Hematology, Shanghai 200025, China</general><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>199912</creationdate><title>Relationship between G-CSF and hyperleukocytosis in patients with APL after treatment with all-trans retinoic acid</title><author>Guosheng, Jiang ; Tianhua, Tang ; Guanlin, Sun ; Yukun, Zhang ; Wen, Wu ; Weili, Wu ; Haiquan, Ren ; Liangyu, Zhao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1904-85a5a3d5a8191bd883056b89ea3aae78756650baab51a1364a37047a1fcea6f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guosheng, Jiang</creatorcontrib><creatorcontrib>Tianhua, Tang</creatorcontrib><creatorcontrib>Guanlin, Sun</creatorcontrib><creatorcontrib>Yukun, Zhang</creatorcontrib><creatorcontrib>Wen, Wu</creatorcontrib><creatorcontrib>Weili, Wu</creatorcontrib><creatorcontrib>Haiquan, Ren</creatorcontrib><creatorcontrib>Liangyu, Zhao</creatorcontrib><collection>CrossRef</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chinese journal of cancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guosheng, Jiang</au><au>Tianhua, Tang</au><au>Guanlin, Sun</au><au>Yukun, Zhang</au><au>Wen, Wu</au><au>Weili, Wu</au><au>Haiquan, Ren</au><au>Liangyu, Zhao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relationship between G-CSF and hyperleukocytosis in patients with APL after treatment with all-trans retinoic acid</atitle><jtitle>Chinese journal of cancer research</jtitle><date>1999-12</date><risdate>1999</risdate><volume>11</volume><issue>4</issue><spage>249</spage><epage>252</epage><pages>249-252</pages><issn>1000-9604</issn><eissn>1993-0631</eissn><abstract>R73; Objective: To detect the G-CSF effect and its mechanism in hyperleucocytosis of patients with APL after treatment with all-trans retinoic acid (ATRA).Methods: MTT method was used to measure the proliferation of HL-60 cells or primary APL cells.cDNA-mRNA dot blot hybridization was used to detect the c-myc gene expression level of HL-60 cells after ATRA treatment. Cycle shift of HL-60 cells was measured by flow cytometric Analysis. Results: ATRA could inhibit obviously in vitro the proliferation of HL60 cells of primary APL cells. The G0/G1 ratio of HL-60at 24 hours or 96 hours post-treatment with ATRA was increased, proliferation of HL-60 cells at the same time was promoted obviously under the condition of rhGCSF. C-myc gene expression was analyzed by dot blot hybridization; its expression levels of 8, 24 or 96 hours in culture with ATRA separately were higher, low, high or lower as compared with each other. Conclusion: GCSF plays an important role in hyperleucocytosis of patients with APL after treatment with ATRA.</abstract><pub>Shangdong Academy of Medical Sciences, Jinan 250062, China%Rui-Jin Hospital, Shanghai Institute of Hematology, Shanghai 200025, China</pub><doi>10.1007/s11670-999-0026-7</doi><tpages>4</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1000-9604 |
ispartof | Chinese journal of cancer research, 1999-12, Vol.11 (4), p.249-252 |
issn | 1000-9604 1993-0631 |
language | eng |
recordid | cdi_wanfang_journals_zgazyj199904004 |
source | SpringerLink Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
title | Relationship between G-CSF and hyperleukocytosis in patients with APL after treatment with all-trans retinoic acid |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-20T10%3A51%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wanfang_jour_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Relationship%20between%20G-CSF%20and%20hyperleukocytosis%20in%20patients%20with%20APL%20after%20treatment%20with%20all-trans%20retinoic%20acid&rft.jtitle=Chinese%20journal%20of%20cancer%20research&rft.au=Guosheng,%20Jiang&rft.date=1999-12&rft.volume=11&rft.issue=4&rft.spage=249&rft.epage=252&rft.pages=249-252&rft.issn=1000-9604&rft.eissn=1993-0631&rft_id=info:doi/10.1007/s11670-999-0026-7&rft_dat=%3Cwanfang_jour_cross%3Ezgazyj199904004%3C/wanfang_jour_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_wanfj_id=zgazyj199904004&rfr_iscdi=true |