Expression of liver insulin-like growth factor 1 gene and its serum level in patients with diabetes
AIM:To explore the effect of diabetic duration and blood glucose level on insulin like growth factor 1(IGF-1) gene expression and serum IGF-1 level.METHODS: Diabetes was induced into Sprague Dawley rats by alloxan and then the rats were subdivided into different groups with varying blood glucose lev...
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| description | AIM:To explore the effect of diabetic duration and blood glucose level on insulin like growth factor 1(IGF-1) gene expression and serum IGF-1 level.METHODS: Diabetes was induced into Sprague Dawley rats by alloxan and then the rats were subdivided into different groups with varying blood glucose level and diabetic duration.The parameters were measured as follows:IGF-1 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR),IGF-1 peptide and serum IGF-1 concentration by enzymelinked immunosorbent assay (ELISA).RESULTS:During early diabetic stage (week 2),in comparison with normal control group (NC), IGF-1 mRNA(1.17±0.069 vs 0.79±0.048, P |
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| fullrecord | <record><control><sourceid>wanfang_jour_proqu</sourceid><recordid>TN_cdi_wanfang_journals_wjg200402022</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>9461708</cqvip_id><wanfj_id>wjg200402022</wanfj_id><sourcerecordid>wjg200402022</sourcerecordid><originalsourceid>FETCH-LOGICAL-c262t-60d58770ebf74bce2b6c7bb02c4829a6404a5e983f49def2b02f3b73faccbf4d3</originalsourceid><addsrcrecordid>eNo1kEFP3DAQhS0Egi3tlSMyEuotW2fsxM4RIWgrIfVCz5HtjIMXr7PYyW7597W0cBrNzPfmjR4hVzVbcynUj8NmXO9L42ENTXNCVgB1V4ES7JSsasZk1XGQF-RLzhvGgPMGzslFLWTdKi5WxD782yXM2U-RTo4Gv8dEfcxL8LEK_hXpmKbD_EKdtvOUaE1HjEh1HKifM82Yli0NuMdQVHSnZ4-xzA--SAavDc6Yv5Izp0PGbx_1kvx9fHi-_1U9_fn5-_7uqbLQwly1bGiUlAyNk8JYBNNaaQwDKxR0uhVM6AY7xZ3oBnRQNo4byctn1jgx8Etye7x70NHpOPabaUmxOPYlJWBMMGAABft-xHZpelswz_3WZ4sh6IjTknvFWFe3oAp4_QEuZotDv0t-q9N7_5leAW6OgH2Z4vjmi6XR9tX5gH0n2loyxf8D_PJ9vw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>80091628</pqid></control><display><type>article</type><title>Expression of liver insulin-like growth factor 1 gene and its serum level in patients with diabetes</title><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Li, Jian-Bo ; Wang, Cheng-Ya ; Chen, Jia-Wei ; Feng, Zhen-Qing ; Ma, Hong-Tai</creator><creatorcontrib>Li, Jian-Bo ; Wang, Cheng-Ya ; Chen, Jia-Wei ; Feng, Zhen-Qing ; Ma, Hong-Tai</creatorcontrib><description><![CDATA[AIM:To explore the effect of diabetic duration and blood glucose level on insulin like growth factor 1(IGF-1) gene expression and serum IGF-1 level.METHODS: Diabetes was induced into Sprague Dawley rats by alloxan and then the rats were subdivided into different groups with varying blood glucose level and diabetic duration.The parameters were measured as follows:IGF-1 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR),IGF-1 peptide and serum IGF-1 concentration by enzymelinked immunosorbent assay (ELISA).RESULTS:During early diabetic stage (week 2),in comparison with normal control group (NC), IGF-1 mRNA(1.17±0.069 vs 0.79±0.048, P<0.001;1.17±0.069 vs 0.53±0.023, P<0.0005, respectively), IGF-1 peptide contents[(196.66±14.9)ng·mg^-1 vs(128.2±11.25)ng·mg^-1,P<0.0005;(196.66±14.9)ng·mg^-1 vs(74.43±5.33)ng·mg^-1, P<0.0001,respectively] were reduced in liver tissues of diabetic rats.The IGF-1 gene downregulation varied with glucose control level of the diabetic state,and deteriorated gradually further with duration of diabetes.By month 6, hepatic tissue IGF-1mRNA was 0.71±0.024 vs 1.12±0.056,P<0.001;0.47±0.021 vs 1.12±0.056, P<0.0005,respectively.IGF-1 peptide was (114.35±8.09) ng·mg^-1 vs (202.05±15.73)ng·mg^-1,P<0.0005; (64.58±3.89) ng·mg^-1 vs(202.05±15.73)ng·mg^-1,P<0.0001 respectively. Serum IGF-1 was also lowered in diabetic group with poor control of blood glucose.On week 2, serum IGF-1 concentrations were (371.0±12.5)ng·mg^-1 vs(511.2±24.7)ng·mg^-1,P<0.0005, (223.2±9.39)ng·mg^-1 vs(511.2±24.7)ng·mg^-1, P<0.0001 respectively. By month 6, (349.6±18.62)ng·mg^-1 vs(520.7±26.32)ng·mg^-1,P<0.0005, (188.5±17.35 vs520.7±26.32)ng·mg^-1,P<0.0001,respectively. Serum IGF-1 peptide change was significantly correlated with that in liver tissue (r=-0.99, P<0.001).Furthermore,No difference was found in the above parameters between diabetic rats with euglycemia and non-diabetic control group.CONCLUSION: The influence of diabetic status on IGF-1 gene expression in liver tissues is started from early diabetic stage, causing down regulation of IGF-1 expression,and progresses with the severity and duration of diabetic state.Accordingly serum IGF-1 level decreases. This might indicate that liver tissue IGF-1 gene expression is greatly affected in diabetes, thus contributing to reduction of serum IGF-1 level.]]></description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v10.i2.255</identifier><identifier>PMID: 14716834</identifier><language>eng</language><publisher>United States: Department of Endocrinology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029,Jiangsu Province, China%Molecular Laboratory, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province,China%Department of Pathology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China</publisher><subject>Animals ; Blood Glucose ; Diabetes Mellitus, Experimental - metabolism ; Diabetes Mellitus, Experimental - physiopathology ; Disease Models, Animal ; ELISA ; Gene Expression - physiology ; Humans ; Insulin-Like Growth Factor I - genetics ; Insulin-Like Growth Factor I - metabolism ; Liver - physiology ; Rats ; Rats, Sprague-Dawley ; RNA, Messenger - analysis ; 动物模型 ; 基因表达 ; 糖尿病 ; 胰岛素样生长因子1 ; 血清学检查 ; 逆转录聚合酶链反应</subject><ispartof>World journal of gastroenterology : WJG, 2004-01, Vol.10 (2), p.255-259</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84123X/84123X.jpg</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14716834$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Jian-Bo</creatorcontrib><creatorcontrib>Wang, Cheng-Ya</creatorcontrib><creatorcontrib>Chen, Jia-Wei</creatorcontrib><creatorcontrib>Feng, Zhen-Qing</creatorcontrib><creatorcontrib>Ma, Hong-Tai</creatorcontrib><title>Expression of liver insulin-like growth factor 1 gene and its serum level in patients with diabetes</title><title>World journal of gastroenterology : WJG</title><addtitle>World Journal of Gastroenterology</addtitle><description><![CDATA[AIM:To explore the effect of diabetic duration and blood glucose level on insulin like growth factor 1(IGF-1) gene expression and serum IGF-1 level.METHODS: Diabetes was induced into Sprague Dawley rats by alloxan and then the rats were subdivided into different groups with varying blood glucose level and diabetic duration.The parameters were measured as follows:IGF-1 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR),IGF-1 peptide and serum IGF-1 concentration by enzymelinked immunosorbent assay (ELISA).RESULTS:During early diabetic stage (week 2),in comparison with normal control group (NC), IGF-1 mRNA(1.17±0.069 vs 0.79±0.048, P<0.001;1.17±0.069 vs 0.53±0.023, P<0.0005, respectively), IGF-1 peptide contents[(196.66±14.9)ng·mg^-1 vs(128.2±11.25)ng·mg^-1,P<0.0005;(196.66±14.9)ng·mg^-1 vs(74.43±5.33)ng·mg^-1, P<0.0001,respectively] were reduced in liver tissues of diabetic rats.The IGF-1 gene downregulation varied with glucose control level of the diabetic state,and deteriorated gradually further with duration of diabetes.By month 6, hepatic tissue IGF-1mRNA was 0.71±0.024 vs 1.12±0.056,P<0.001;0.47±0.021 vs 1.12±0.056, P<0.0005,respectively.IGF-1 peptide was (114.35±8.09) ng·mg^-1 vs (202.05±15.73)ng·mg^-1,P<0.0005; (64.58±3.89) ng·mg^-1 vs(202.05±15.73)ng·mg^-1,P<0.0001 respectively. Serum IGF-1 was also lowered in diabetic group with poor control of blood glucose.On week 2, serum IGF-1 concentrations were (371.0±12.5)ng·mg^-1 vs(511.2±24.7)ng·mg^-1,P<0.0005, (223.2±9.39)ng·mg^-1 vs(511.2±24.7)ng·mg^-1, P<0.0001 respectively. By month 6, (349.6±18.62)ng·mg^-1 vs(520.7±26.32)ng·mg^-1,P<0.0005, (188.5±17.35 vs520.7±26.32)ng·mg^-1,P<0.0001,respectively. Serum IGF-1 peptide change was significantly correlated with that in liver tissue (r=-0.99, P<0.001).Furthermore,No difference was found in the above parameters between diabetic rats with euglycemia and non-diabetic control group.CONCLUSION: The influence of diabetic status on IGF-1 gene expression in liver tissues is started from early diabetic stage, causing down regulation of IGF-1 expression,and progresses with the severity and duration of diabetic state.Accordingly serum IGF-1 level decreases. This might indicate that liver tissue IGF-1 gene expression is greatly affected in diabetes, thus contributing to reduction of serum IGF-1 level.]]></description><subject>Animals</subject><subject>Blood Glucose</subject><subject>Diabetes Mellitus, Experimental - metabolism</subject><subject>Diabetes Mellitus, Experimental - physiopathology</subject><subject>Disease Models, Animal</subject><subject>ELISA</subject><subject>Gene Expression - physiology</subject><subject>Humans</subject><subject>Insulin-Like Growth Factor I - genetics</subject><subject>Insulin-Like Growth Factor I - metabolism</subject><subject>Liver - physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Messenger - analysis</subject><subject>动物模型</subject><subject>基因表达</subject><subject>糖尿病</subject><subject>胰岛素样生长因子1</subject><subject>血清学检查</subject><subject>逆转录聚合酶链反应</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kEFP3DAQhS0Egi3tlSMyEuotW2fsxM4RIWgrIfVCz5HtjIMXr7PYyW7597W0cBrNzPfmjR4hVzVbcynUj8NmXO9L42ENTXNCVgB1V4ES7JSsasZk1XGQF-RLzhvGgPMGzslFLWTdKi5WxD782yXM2U-RTo4Gv8dEfcxL8LEK_hXpmKbD_EKdtvOUaE1HjEh1HKifM82Yli0NuMdQVHSnZ4-xzA--SAavDc6Yv5Izp0PGbx_1kvx9fHi-_1U9_fn5-_7uqbLQwly1bGiUlAyNk8JYBNNaaQwDKxR0uhVM6AY7xZ3oBnRQNo4byctn1jgx8Etye7x70NHpOPabaUmxOPYlJWBMMGAABft-xHZpelswz_3WZ4sh6IjTknvFWFe3oAp4_QEuZotDv0t-q9N7_5leAW6OgH2Z4vjmi6XR9tX5gH0n2loyxf8D_PJ9vw</recordid><startdate>20040115</startdate><enddate>20040115</enddate><creator>Li, Jian-Bo</creator><creator>Wang, Cheng-Ya</creator><creator>Chen, Jia-Wei</creator><creator>Feng, Zhen-Qing</creator><creator>Ma, Hong-Tai</creator><general>Department of Endocrinology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029,Jiangsu Province, China%Molecular Laboratory, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province,China%Department of Pathology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20040115</creationdate><title>Expression of liver insulin-like growth factor 1 gene and its serum level in patients with diabetes</title><author>Li, Jian-Bo ; Wang, Cheng-Ya ; Chen, Jia-Wei ; Feng, Zhen-Qing ; Ma, Hong-Tai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c262t-60d58770ebf74bce2b6c7bb02c4829a6404a5e983f49def2b02f3b73faccbf4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Blood Glucose</topic><topic>Diabetes Mellitus, Experimental - metabolism</topic><topic>Diabetes Mellitus, Experimental - physiopathology</topic><topic>Disease Models, Animal</topic><topic>ELISA</topic><topic>Gene Expression - physiology</topic><topic>Humans</topic><topic>Insulin-Like Growth Factor I - genetics</topic><topic>Insulin-Like Growth Factor I - metabolism</topic><topic>Liver - physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Messenger - analysis</topic><topic>动物模型</topic><topic>基因表达</topic><topic>糖尿病</topic><topic>胰岛素样生长因子1</topic><topic>血清学检查</topic><topic>逆转录聚合酶链反应</topic><toplevel>online_resources</toplevel><creatorcontrib>Li, Jian-Bo</creatorcontrib><creatorcontrib>Wang, Cheng-Ya</creatorcontrib><creatorcontrib>Chen, Jia-Wei</creatorcontrib><creatorcontrib>Feng, Zhen-Qing</creatorcontrib><creatorcontrib>Ma, Hong-Tai</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Jian-Bo</au><au>Wang, Cheng-Ya</au><au>Chen, Jia-Wei</au><au>Feng, Zhen-Qing</au><au>Ma, Hong-Tai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of liver insulin-like growth factor 1 gene and its serum level in patients with diabetes</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World Journal of Gastroenterology</addtitle><date>2004-01-15</date><risdate>2004</risdate><volume>10</volume><issue>2</issue><spage>255</spage><epage>259</epage><pages>255-259</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract><![CDATA[AIM:To explore the effect of diabetic duration and blood glucose level on insulin like growth factor 1(IGF-1) gene expression and serum IGF-1 level.METHODS: Diabetes was induced into Sprague Dawley rats by alloxan and then the rats were subdivided into different groups with varying blood glucose level and diabetic duration.The parameters were measured as follows:IGF-1 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR),IGF-1 peptide and serum IGF-1 concentration by enzymelinked immunosorbent assay (ELISA).RESULTS:During early diabetic stage (week 2),in comparison with normal control group (NC), IGF-1 mRNA(1.17±0.069 vs 0.79±0.048, P<0.001;1.17±0.069 vs 0.53±0.023, P<0.0005, respectively), IGF-1 peptide contents[(196.66±14.9)ng·mg^-1 vs(128.2±11.25)ng·mg^-1,P<0.0005;(196.66±14.9)ng·mg^-1 vs(74.43±5.33)ng·mg^-1, P<0.0001,respectively] were reduced in liver tissues of diabetic rats.The IGF-1 gene downregulation varied with glucose control level of the diabetic state,and deteriorated gradually further with duration of diabetes.By month 6, hepatic tissue IGF-1mRNA was 0.71±0.024 vs 1.12±0.056,P<0.001;0.47±0.021 vs 1.12±0.056, P<0.0005,respectively.IGF-1 peptide was (114.35±8.09) ng·mg^-1 vs (202.05±15.73)ng·mg^-1,P<0.0005; (64.58±3.89) ng·mg^-1 vs(202.05±15.73)ng·mg^-1,P<0.0001 respectively. Serum IGF-1 was also lowered in diabetic group with poor control of blood glucose.On week 2, serum IGF-1 concentrations were (371.0±12.5)ng·mg^-1 vs(511.2±24.7)ng·mg^-1,P<0.0005, (223.2±9.39)ng·mg^-1 vs(511.2±24.7)ng·mg^-1, P<0.0001 respectively. By month 6, (349.6±18.62)ng·mg^-1 vs(520.7±26.32)ng·mg^-1,P<0.0005, (188.5±17.35 vs520.7±26.32)ng·mg^-1,P<0.0001,respectively. Serum IGF-1 peptide change was significantly correlated with that in liver tissue (r=-0.99, P<0.001).Furthermore,No difference was found in the above parameters between diabetic rats with euglycemia and non-diabetic control group.CONCLUSION: The influence of diabetic status on IGF-1 gene expression in liver tissues is started from early diabetic stage, causing down regulation of IGF-1 expression,and progresses with the severity and duration of diabetic state.Accordingly serum IGF-1 level decreases. This might indicate that liver tissue IGF-1 gene expression is greatly affected in diabetes, thus contributing to reduction of serum IGF-1 level.]]></abstract><cop>United States</cop><pub>Department of Endocrinology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029,Jiangsu Province, China%Molecular Laboratory, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province,China%Department of Pathology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China</pub><pmid>14716834</pmid><doi>10.3748/wjg.v10.i2.255</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Blood Glucose Diabetes Mellitus, Experimental - metabolism Diabetes Mellitus, Experimental - physiopathology Disease Models, Animal ELISA Gene Expression - physiology Humans Insulin-Like Growth Factor I - genetics Insulin-Like Growth Factor I - metabolism Liver - physiology Rats Rats, Sprague-Dawley RNA, Messenger - analysis 动物模型 基因表达 糖尿病 胰岛素样生长因子1 血清学检查 逆转录聚合酶链反应 |
| title | Expression of liver insulin-like growth factor 1 gene and its serum level in patients with diabetes |
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