Expression of liver insulin-like growth factor 1 gene and its serum level in patients with diabetes

AIM:To explore the effect of diabetic duration and blood glucose level on insulin like growth factor 1(IGF-1) gene expression and serum IGF-1 level.METHODS: Diabetes was induced into Sprague Dawley rats by alloxan and then the rats were subdivided into different groups with varying blood glucose level and diabetic duration.The parameters were measured as follows:IGF-1 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR),IGF-1 peptide and serum IGF-1 concentration by enzymelinked immunosorbent assay (ELISA).RESULTS:During early diabetic stage (week 2),in comparison with normal control group (NC), IGF-1 mRNA(1.17±0.069 vs 0.79±0.048, P