Autotoxicity of Phthalate Esters in Tobacco Root Exudates：Effects on Seed Germination and Seedling Growth

Autotoxicity is one of the major factors that impede continuous cropping.It is defined as the toxic influence of chemicals released from one plant species on the germination and growth of individuals of the same species.Here, in order to exam the autotoxicity of tobacco root exudates, root exudates were collected from tobacco plants grown both in cultural solution and on natural soil.Using ultraperformance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry, main autotoxic chemical substances in the root exudates were identified.The autotoxic effects of suspected autotoxins on seed germination（including germination rate,germination potential, germination index, and vigor index） and seedling growth were analyzed.Dibutyl phthalate（or diisobutyl phthalate）, dioctyl phthalate, and diisooctyl phthalate were identified in tobacco root exudates.It was observed that high concentrations（greater than 0.5 mmol L~（-1）） of each identified phthalate ester caused significant（P 〈 0.05） inhibition of tobacco seed germination and seedling growth.It can be concluded that phthalate esters such as dibutyl phthalate, diisobutyl phthalate, and diisooctyl phthalate in tobacco root exudates may play an important role in tobacco autotoxicity.